Gut microbiota mediates the protective effects of dietary β-hydroxy-β-methylbutyrate (HMB) against obesity induced by high-fat diets
Gut microbiota mediates the protective effects of dietary β-hydroxy-β-methylbutyrate (HMB) against obesity induced by high-fat diets
复制标题
肠道微生物群介导膳食β-羟基-β-甲基丁酸酯（HMB）对高脂肪饮食引起的肥胖的保护作用
DOI：
10.1096/fj.201900665rr
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发表时间：
2019-09-01
期刊：
影响因子：
4.8
通讯作者：
Kong, Xiangfeng
中科院分区：
文献类型：
--
作者：
Duan, Yehui;Zhong, Yinzhao;Kong, Xiangfeng
肥胖会增加患胰岛素抵抗和糖尿病的风险，这是一个主要的公共卫生问题。我们先前的研究表明，饮食β-羟基β-甲基丁酸（HMB）可改善猪模型中的脂质代谢。但是，尚不清楚HMB是否通过肠道菌群阻止肥胖症。在这项研究中，我们发现HMB减轻了体重，减轻了棕色脂肪组织的美白，并改善了喂养高脂饮食（HFD）的小鼠的胰岛素抵抗。 16S rRNA的高通量旋转测序表明，HMB给药可显着逆转HFD喂养的小鼠的肠道微生物群营养不良，包括肠道微生物群的多样性以及细菌植物和公司的相对丰度。此外，从HMB处理的小鼠中移植了微生物群，减弱了HFD诱导的脂质代谢疾病。此外，HFD喂养的小鼠显示出较低的短链脂肪酸，而HMB的给药增加了丙酸的产生。相关分析确定了丙酸的产生与相对细菌的丰度之间的显着相关性。丙酸钠治疗还减弱了HFD诱导的脂质代谢疾病。总的来说，我们的结果表明，HMB可能被用作益生菌剂逆转HFD诱导的肥胖症，并且潜在的机制与重编程肠道菌群和代谢相关，尤其是杀菌剂介导的丙酸产生。在未来的研究中，应采取更多的努力来确认和扩展HMB对人类模型的有益影响。Duan，Y.，Y.，Y.，Y.，Xiao，H.，Zheng，C.，Song，B.，Wang，Wang，Wang， W.，Guo，Q.，Li，Y.，Han，H.，Gao，J.，Xu，K.，Li，T.，Yin，Y. X.肠道微生物群介导饮食β-羟基-Beta-甲基丁酸（HMB）对高脂饮食引起的肥胖症的保护作用。
Obesity increases the risk of developing insulin resistance and diabetes and is a major public health concern. Our previous study shows that dietary beta-hydroxy-beta-methylbutyrate (HMB) improves lipid metabolism in a pig model. However, it remains unclear whether HMB blocks obesity through gut microbiota. In this study, we found that HMB reduced body weight, alleviated the whitening of brown adipose tissue, and improved insulin resistance in mice fed a high-fat diet (HFD). High-throughput pyrosequencing of the 16S rRNA demonstrated that HMB administration significantly reversed the gut microbiota dysbiosis in HFD-fed mice, including the diversity of gut microbiota and relative abundances of Bacteroidetes and Firmicutes. Moreover, microbiota transplantation from HMB-treated mice attenuated HFD-induced lipid metabolic disorders. Furthermore, HFD-fed mice showed lower short-chain fatty acids, whereas administration of HMB increased the propionic acid production. Correlation analysis identified a significant correlation between propionic acid production and the relative Bacteroidetes abundance. Sodium propionate treatment also attenuated HFD-induced lipid metabolic disorders. Collectively, our results indicated that HMB might be used as a probiotic agent to reverse HFD-induced obesity, and the potential mechanism was associated with reprogramming gut microbiota and metabolism, especially Bacteroidetes-mediated propionic acid production. In future studies, more efforts should be made to confirm and expand the beneficial effects of HMB to human models.-Duan, Y., Zhong, Y., Xiao, H., Zheng, C., Song, B., Wang, W., Guo, Q., Li, Y., Han, H., Gao, J., Xu, K., Li, T., Yin, Y., Li, F., Yin, J., Kong, X. Gut microbiota mediates the protective effects of dietary beta-hydroxy-beta-methylbutyrate (HMB) against obesity induced by high-fat diets.