Non-invasive Amino Acid Profiling of Embryo Culture Medium Using HPLC Correlates With Embryo Implantation Potential in Women Undergoing in vitro Fertilization
Non-invasive Amino Acid Profiling of Embryo Culture Medium Using HPLC Correlates With Embryo Implantation Potential in Women Undergoing in vitro Fertilization
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使用 HPLC 对胚胎培养基进行无创氨基酸分析与体外受精女性的胚胎着床潜力相关
DOI：
10.3389/fphys.2020.00405
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发表时间：
2020-05-20
影响因子：
4
通讯作者：
Zhang, Shun
中科院分区：
文献类型：
--
作者：
Huo, Peng;Zhu, Yunshan;Zhang, Shun
本研究旨在确定体外受精（IVF）妇女3日龄胚胎培养液的氨基酸谱与胚胎植入潜力之间的相关性。回顾性分析2015年12月至2017年2月在我院接受试管婴儿治疗的98例患者的资料。将98例患者分为妊娠组（双胎妊娠）、非妊娠组（非妊娠）和空白对照组。收集来自3日龄胚胎培养基和空白对照培养基的氨基酸，并使用高效液相色谱法（HPLC）进行分析。结果表明，在3日龄胚胎培养液和空白对照培养液中检测到天冬氨酸（ASP）、丝氨酸（SER）、甘氨酸（GLY）、组氨酸（HIS）、牛磺酸（TAU）、精氨酸（ARG）、苏氨酸（THR）、丙氨酸（ALA）和脯氨酸（PRO）等氨基酸。妊娠组与非妊娠组在峰高（H）-SER、表面积（S）-ASP、S-SER、S-HIS和S-ALA上有显著性差异。根据氨基酸峰高和峰面积进行判别分析，妊娠组、非妊娠组和空白对照组的预测率分别为82.7%、95.7%和100%。此外，通过使用主成分分析，我们发现这三组的预测率分别为90.4%，91.3%和100%。本研究结果提示，利用氨基酸浓度进行主成分分析和判别分析，可以准确预测氨基酸指纹图谱与胚胎着床潜能的关系。
This study aimed to determine the correlation between amino acid profiling of a 3-day-old embryo culture medium and embryo implantation potential in women undergoing in vitro fertilization (IVF). The data of 98 patients who received IVF treatment in our hospital from December 2015 to February 2017 were retrospectively analyzed. The 98 patients were grouped into a pregnant group (gemellary pregnancy), a non-pregnant group (non-pregnancy), and a blank control group. The amino acids from a 3-day-old embryo culture medium and blank control medium were collected and were analyzed using high performance liquid chromatography (HPLC). The HPLC results showed that amino acids including aspartate (ASP), serine (SER), glycine (GLY), histidine (HIS), taurine (TAU), arginine (ARG), threonine (THR), alanine (ALA), and proline (PRO) were detected in the 3-day-old embryo culture medium and blank control medium. There are significant differences between the pregnant group and non-pregnant group in peak height (H)-SER, surface area (S)-ASP, S-SER, S-HIS, and S-ALA. The discrimination analysis according to the peak height and peak area of amino acids revealed that the prediction rate of the pregnant group, non-pregnant group, and blank control group were 82.7, 95.7, and 100%. Further, by using the principal component analysis, we found that the prediction rate in these three groups were 90.4, 91.3, and 100%. Our data may suggest that using amino acid concentrations for principal component analysis and discriminant analysis has high accuracy in predicting the relationship between amino acid fingerprint and embryo implantation potential.