Differential regulation of physiological activities by RcsB and OmpR in Yersinia enterocolitica
Differential regulation of physiological activities by RcsB and OmpR in Yersinia enterocolitica
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小肠结肠炎耶尔森氏菌中 RcsB 和 OmpR 生理活性的差异调节。
DOI：
10.1093/femsle/fnz210
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发表时间：
2019-09-01
影响因子：
2.1
通讯作者：
Chen, Jingyu
中科院分区：
文献类型：
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作者：
Meng, Jiao;Bai, Jiaqi;Chen, Jingyu
深入了解Rcs和EnvZ/OmpR磷酸化中继系统的机制，使小肠结肠炎耶尔森菌在各种环境中茁壮成长，是预防和控制小肠结肠炎耶尔森菌的关键。小肠结肠炎在这项研究中，我们发现RcsB和OmpR在调节Y中多种生理过程中具有不同的功能。小肠结肠炎rcsB突变体通过上调flhDC、hmsHFRS和hmsT刺激鞭毛生物合成并增加运动性、生物膜形成和c-di-GMP产生。然而，ompR突变表现出非运动表型，由于缺乏鞭毛。当Y.小肠结肠炎菌暴露于低渗透压条件。此外，OmpR被确定为对Y.小肠结肠炎菌在极端温度条件下生长。重要的是，Y.小肠结肠炎菌对多粘菌素B和SDS的敏感性高于rcs B突变。由于运动性、生物膜形成和环境耐受性对于宿主的细菌定殖是至关重要的，这些发现表明OmpR比RcsB在形成Y的致病表型方面更关键。小肠结肠炎
A thorough understanding of the mechanisms of Rcs and EnvZ/OmpR phosphorelay systems that allow Yersinia enterocolitica to thrive in various environments is crucial to prevent and control Y. enterocolitica infections. In this study, we showed that RcsB and OmpR have the ability to function differently in modulating a diverse array of physiological processes in Y. enterocolitica. The rcsB mutant stimulated flagella biosynthesis and increased motility, biofilm formation and c-di-GMP production by upregulating flhDC, hmsHFRS and hmsT. However, mutation in ompR exhibited a non-motile phenotype due to the lack of flagella. Biofilm formation was reduced and less c-di-GMP was produced through the downregulation of flhDC, hmsHFRS and hmsT expression when Y. enterocolitica was exposed to low osmolarity conditions. Furthermore, OmpR was identified to be important for Y. enterocolitica to grow in extreme temperature conditions. Importantly, ompR mutation in Y. enterocolitica were more sensitive to polymyxin B and SDS than rcsB mutation were. Since motility, biofilm formation and environmental tolerance are critical for bacterial colonization of the host, these findings indicated that OmpR is more critical than RcsB in shaping the pathogenic phenotype of Y. enterocolitica.