A detailed phenotypic characterisation of the type strains of Halomonas species
A detailed phenotypic characterisation of the type strains of Halomonas species
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DOI：
10.1078/0723-2020-00122
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发表时间：
2002-10-01
影响因子：
3.4
通讯作者：
Béjar, V
中科院分区：
文献类型：
--
作者：
Mata, JA;Martínez-Cánovas, J;Béjar, V
我们对盐单胞菌属内21个种的模式菌株进行了详细的表型表征，并研究了盐单胞菌属内菌株的任何可能的种内变异。eurihalina，海鲶H. halophila，盐生H. maura和H. salina。我们使用了234个形态，生理，生化，营养和抗菌药物敏感性试验。营养测定进行了使用经典和小型化（BIOPHASE系统）的识别方法。使用TAXAN程序进行了两种不同的数值分析;第一种包括所有测试的差异数据，而第二种仅使用诊断分数最高的57个测试（！0.5）。这两种分析的结果是非常相似的，并证明了表型异质性的盐单胞菌物种的问题。在62%的相似性水平上，模式种可分为三个现象，它们之间的主要区别是现象A（H。aquamarina，水刺蛾H. Chelsea，H. cupida，H. pantelleriensis和H.嗜盐菌）从糖中产生酸。该种的类群为C（H. campisalis，山核桃H. desiderata和H. subglasciescola）使用的有机底物比其它的少。其余菌株包括在现象B中。H.马里氏黄素是明显不同的，因此不包括在三种phena中的任何一种中。盐单胞菌campisalis和盐单胞菌desiderata. We的工作结果之间的高表型相似性（超过88%）被发现，应允许研究人员尽量减少所需的测试，以达到一个可靠的表型特征的盐单胞菌菌株，并选择那些最有用的，以区分盐单胞菌物种彼此。
We have made a detailed phenotypic characterisation of the type strains of 21 species within the genus Halomonas and have also studied any possible intraspecific variation of strains within H. eurihalina, H. halophila, H. maura and H. salina. We used 234 morphological, physiological, biochemical, nutritional and antimicrobial susceptibility tests. Nutritional assays were carried out using both classical and miniaturized (BIOLOG system) identification methods. Two different numerical analyses were made using the TAXAN program; the first included the differential data from all the tests carried out whilst the second used only the 57 tests with the highest diagnostic scores ( ! 0.5). The results of both analyses were quite similar and demonstrated the phenotypic heterogeneity of the Halomonas species in question. At a 62% similarity level the type species were grouped into three phena, the main difference between them being the capacity of those included within phenon A (H. aquamarina, H. meridiana, H. cupida, H. pantelleriensis and H. halmophila) to produce acids from sugars. The species grouped in phenon C (H. campisalis, H. desiderata and H. subglasciescola) used fewer organic substrates than the others. The remaining strains were included in phenon B. H. marisflavi was clearly distinct and thus was not included in any of the three phena. High phenotypic similarity (more than 88%) was found between Halomonas campisalis and Halomonas desiderata.The results of our work should allow researchers to minimise the tests required to arrive at a reliable phenotypic characterisation of Halomonas isolates and to select those of most use to differentiate Halomonas species from each other.