CAPS (mammalian UNC-31) protein localizes to membranes involved in dense-core vesicle exocytosis
CAPS (mammalian UNC-31) protein localizes to membranes involved in dense-core vesicle exocytosis
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DOI：
10.1016/s0896-6273(00)80521-8
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发表时间：
1998-07-01
期刊：
影响因子：
16.2
通讯作者：
Martin, TFJ
中科院分区：
文献类型：
--
作者：
Berwin, B;Floor, E;Martin, TFJ
CAPS 是一种神经/内分泌特异性蛋白，被发现是可渗透性神经内分泌细胞中 Ca2+ 激活的致密核心囊泡 (DCV) 胞吐作用所需的胞质因子。我们报告说，CAPS 也是脑匀浆中与膜相关的外周结合蛋白，选择性地定位于质膜和 DCV，但不定位于小的透明突触小泡 (SV)。 CAPS 通过与双层磷脂相互作用，对 DCV 表现出高亲和力和可饱和结合。特异性 CAPS 抗体可抑制 Ca2+ 激活的去甲肾上腺素从含有膜相关 CAPS 的裂解突触体中释放，表明膜结合 CAPS 对于神经 DCV 胞吐作用至关重要。 CAPS 是胞吐机制的一个功能组件，选择性地定位于 DCV，并且它可能赋予神经肽和生物胺递质分泌不同的调节特征。
CAPS is a neural/endocrine-specific protein discovered as a cytosolic factor required for Ca2+-activated dense-core vesicle (DCV) exocytosis in permeable neuroendocrine cells. We report that CAPS is also a membrane-associated, peripherally bound protein in brain homogenates that localizes selectively to plasma membranes and to DCVs but not to small clear synaptic vesicles (SVs). CAPS exhibits high affinity and saturable binding to DCVs by interaction with bilayer phospholipids. Specific CAPS antibodies inhibit Ca2+-activated norepinephrine release from lysed synaptosomes that contain membrane-associated CAPS, indicating that membrane-bound CAPS is essential for neural DCV exocytosis. CAPS is a functional component of the exocytotic machinery that localizes selectively to DCVs, and it may confer distinct regulatory features on neuropeptide and biogenic amine transmitter secretion.