Butyrate production from oligofructose fermentation by the human faecal flora: what is the contribution of extracellular acetate and lactate?
Butyrate production from oligofructose fermentation by the human faecal flora: what is the contribution of extracellular acetate and lactate?
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DOI：
10.1079/bjn20061853
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发表时间：
2006-09-01
影响因子：
3.6
通讯作者：
Weaver, Lawrence T.
中科院分区：
文献类型：
--
作者：
Morrison, Douglas J.;Mackay, William G.;Weaver, Lawrence T.
丁酸是维持结肠健康的重要底物，人粪便细菌发酵低聚果糖可提高丁酸的体外产量。然而，低聚果糖似乎主要由产乙酸盐和乳酸盐的细菌而不是产丁酸盐的细菌发酵。使用[U-C-13（6）]葡萄糖进行同位素标记研究，结果表明C-13（2）和C-13（4）是葡萄糖发酵产生的主要标记丁酸盐，通过[C-13（2）]乙酸-乙酰CoA作为中间体。定量细菌相互转化反应，并观察乙酸盐转化为丁酸盐和乳酸盐转化为乙酸盐、丙酸盐和丁酸盐。添加低聚果糖的粪便分批培养显着增加丁酸生产。在由低聚果糖发酵新合成的丁酸盐中，80%来自胞外乙酸盐和乳酸盐的相互转化，其中乙酸盐在数量上更重要。碳水化合物，如低聚果糖，具有益生元特性。此外，低聚果糖选择性地刺激细菌将乙酸和乳酸转化为丁酸。具有类似性质的碳水化合物代表了益生元定义的细化，称为产丁酸益生元，因为它们具有额外的功能。
Butyrate is an important substrate for maintenance of colonic health and oligofructose fermentation by human faecal bacteria can increase butyrate production in vitro. However, oligofructose appears to be fermented by mainly acetate and lactate-producing bacteria rather than butyrate-producing bacteria. Isotope labelling studies using [U-C-13(6)]glucose were used to show that C-13(2) and C-13(4) were the major labelled butyrate species produced from glucose fermentation, via [C-13(2)]acetate-acetyl CoA as intermediate. Bacterial interconversion reactions were quantified and acetate conversion to butyrate and lactate conversion to acetate, propionate and butyrate were observed. Addition of oligofructose to faecal batch cultures significantly increased butyrate production. Of the newly synthesised butyrate from oligofructose fermentation, 80 % was derived from interconversion of extracellular acetate and lactate, with acetate being quantitatively more significant. Carbohydrates, such as oligofructose, have prebiotic properties. In addition, oligofructose selectively stimulates the bacterial conversion of acetate and lactate to butyrate. Carbohydrates with similar properties represent a refinement of the prebiotic definition, termed butyrogenic prebiotics, because of their additional functionality.