CELL FRACTIONATION OF NORMAL AND MALIGNANT TISSUES
CELL FRACTIONATION OF NORMAL AND MALIGNANT TISSUES
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DOI：
10.1016/0014-4827(54)90145-7
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发表时间：
1954-01-01
影响因子：
3.7
通讯作者：
LAIRD, AK
中科院分区：
文献类型：
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作者：
LAIRD, AK
从大鼠、小鼠和人获得用于分级分离的组织。正常大鼠组织包括肝脏、肾脏、胸腺、胰腺和颌下腺;大鼠肿瘤包括Flexner-Jobling癌、步行者256癌肉瘤、詹森肉瘤和三种不同致癌物诱导的肝脏肿瘤。正常小鼠肝脏，和以下小鼠肿瘤进行分级：可移植的乳腺腺癌，可移植的肾上腺癌，和可移植的淋巴肉瘤。一个人肾上腺和两个人肿瘤也被细分：一个纤维肉瘤和一个肾上腺瘤。对于正常组织和原发性大鼠肿瘤的研究，使用Sprague-Dawley品系的雄性白化病大鼠;该品系的雌性大鼠用作可移植大鼠肿瘤的宿主。给它们喂食补充有全谷物饮食和水的市售犬粮，自由采食。在所有情况下，动物在处死前均可获得食物。从雌性罗克兰系白色小鼠中获得小鼠肝脏;将两种性别的C、H小鼠用作乳腺癌和淋巴肉瘤的宿主;将去势Ce或F、杂交Ce小鼠用作肾上腺car-1的宿主。美国公共卫生署和美国癌症协会。它得到了亚历山大和玛格丽特斯图尔特信托基金的部分支持。本文是一篇论文的一部分，该论文部分符合威斯康星州大学动物学系哲学博士学位的要求。
Tissues for fractionation were obtained from rats, mice, and humans. The normal rat tissues included liver, kidney, thymus, pancreas, and submaxillary gland; the tumors from rats included the Flexner-Jobling carcinoma, Walker 256 carcinosarcoma, the Jensen sarcoma, and liver tumors induced by three different carcinogens. Normal mouse liver, and the following mouse tumors were fractionated: a transplantable mammary adenocarcinoma, a transplantable adrenal carcinoma, and a transplantable lymphosarcoma. A human adrenal gland and two human tumors were also fractionated: a fibrosarcoma and a hypernephroma. For the study of normal tissues and of primary rat tumors, male albino rats of the Sprague-Dawley strain were used; female rats of this strain were used as hosts for the transplantable rat tumors. They were fed a commercial dog chow supplemented with a whole grain diet, and water, ad libitum. Food in all cases was available to the animals until the time they were killed. The mouse livers were obtained from female Rockland strain white mice; C, H mice of both sexes were used as hosts for the mammary adenocarcinoma and lymphosarcoma; castrated Ce or F, hybrid Ce mice were the hosts for the adrenal car-1 This work was carried out during tenure of fellowships of the National Cancer Institute. US Public Health Service, and of the American Cancer Society. It was supported in part by the Alexander and Margaret Stewart Trust. This paper is part of a thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Zoology at the University of Wisconsin.