Activity of growth hormone peptides bGH 96-133 and hGH 95-133 in 3T3-F442A cells.
Activity of growth hormone peptides bGH 96-133 and hGH 95-133 in 3T3-F442A cells.
复制标题
3T3-F442A 细胞中生长激素肽 bGH 96-133 和 hGH 95-133 的活性。
DOI：
10.1016/0303-7207(94)90008-6
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发表时间：
1994
影响因子：
4.1
通讯作者：
Allen,DL
中科院分区：
文献类型：
--
作者：
Sonenberg,M;Guller,S;Wu,KY;Corin,RE;Allen,DL
化学合成的牛生长激素（bGH）bGH 96-133及其人同系物hGH 95-133具有相似的体外生物学活性。与天然GH不同，bGH 96-133和hGH 95-133在高达10 μM的剂量下完全没有脂肪形成或抗胰岛素活性。bGH 96-133具有胰岛素样活性，10 μM时葡萄糖摄取增加100%。bGH具有抗促有丝分裂作用，bGH 96-133和hGH 95-133具有促有丝分裂作用（EC 50 ~ 180 nM，最大反应浓度为1-2 μM）。仅bGH 96-133和hGH 95-133取代了3 T3-F442 A成纤维细胞与[125 I]hGH 95-133的结合，aKd在60-120 nM之间。bGH、hGH、胰岛素和IGF-I对[125 I]hGH 95-133结合无影响。bGH 96-133和hGH 95-133未显著抑制[125 I]hGH或[125 I]IGF-I结合。这些实验表明，含有GH的肽bGH 96-133和hGH 95-133具有促有丝分裂和胰岛素样活性，而没有bGH的脂肪形成、抗胰岛素或抗促有丝分裂活性。这些肽具有特异性结合位点，其似乎不同于GH、胰岛素和IGF-I受体。
Chemically synthesized bovine growth hormone (bGH) bGH 96–133 and its human homologue, hGH 95–133, have similar in vitro biological activities. Unlike native GH, bGH 96–133 and hGH 95–133 were completely without adipogenic or anti-insulin activity at doses up to 10 μM. bGH 96–133 had insulin-like activity, with a 100% increase in glucose uptake at 10 μM. bGH was anti-mitogenic and bGH 96–133 and hGH 95–133 were mitogenic (EC50~ 180 nM and maximal response at 1–2 μM). Only bGH 96–133 and hGH 95–133 displaced [125I]hGH 95–133 binding from 3T3-F442A fibroblasts with aKdbetween 60–120 nM. bGH, hGH, insulin and IGF-I were without effect on [125I]hGH 95–133 binding. bGH 96–133 and hGH 95–133 did not significantly inhibit [125I]hGH or [125I]IGF-I binding. These experiments indicate that GH containing peptides bGH 96–133 and hGH 95–133 have mitogenic and insulin-like activity without the adipogenic, anti-insulin or anti-mitogenic activity of bGH. These peptides have a specific binding site which appears to be distinct from the GH, insulin and IGF-I receptors.