A novel technique for propagation of Porphyra yezoensis Ueda blades in suspension cultures via monospores
A novel technique for propagation of Porphyra yezoensis Ueda blades in suspension cultures via monospores
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DOI：
10.1023/a:1008183402730
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发表时间：
1999-01-01
影响因子：
3.3
通讯作者：
Hafting, JT
中科院分区：
文献类型：
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作者：
Hafting, JT
现有的紫菜叶片悬浮培养繁殖方法不适合商业化生产。对条斑紫菜叶片悬浮培养繁殖的新方法进行了试验。通过将各种大小的叶片切割成不同大小的组织切片并将这些切片置于新鲜培养基中，经由悬浮液中的单孢子无性繁殖叶片。2-3周后，切片完全崩解，产生单孢子，然后是叶片的致密悬浮培养物。这种技术显示出很大的希望，从一个初始叶片接种物生产多种作物，提高产量，并简化了这种非碎片化的繁殖，在陆基水箱海水养殖。
The present methods for propagation of Porphyra blades in suspension cultures are inadequate for commercial production. A novel method for propagation of P. yezoensis blades in suspension cultures was tested. Blades were asexually propagated via monospores in suspension by cutting blades of various sizes into differently sized tissue sections and inoculating these in fresh medium. After 2-3 weeks, the sections completely disintegrated, producing monospores followed by a dense suspension culture of blades. This technique shows great promise for producing multiple crops from one initial blade inoculum, increasing production, and simplifying the propagation of this non-fragmenting alga in land-based tank mariculture.