3D image reconstruction of histopathological structure of atherosclerotic plaque using a novel technique with film tomography
3D image reconstruction of histopathological structure of atherosclerotic plaque using a novel technique with film tomography
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使用胶片断层扫描新技术重建动脉粥样硬化斑块的组织病理学结构的 3D 图像
DOI：
10.1016/j.jcmg.2014.04.028
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
S. Noji and A. Hirayama
中科院分区：
文献类型：
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作者：
T. Hiro;T. Takayama;H. Haruta;M. Mitsumori;K. Tanaka;J. Kawanabe;S. Noji and A. Hirayama
2014年12月：1280-8 1283或光学相干断层扫描是流行的。然而，这些技术在确定斑块的组织学结构方面仍然存在局限性，组织学技术的进步可能会增强病理学研究的贡献。Mitsumori等人(1)已经开发了一种称为“胶片断层摄影术”的技术，用粘性薄膜制备组织切片。自动切片机可将石蜡包埋的组织块连续切成6 μ m厚的切片（1 cm长的组织样本约1，600张载玻片），然后将切片连续放置在长胶片卷上。将切片自动放置在塑料框架中，可以用常规的病理或免疫组化染色进行处理。这是首次将该技术应用于死后人冠状动脉粥样硬化的三维评估。连续切片可以被重建为纵向切片、整个血管体的外部成像、用于描绘组织分布的半透明整个血管成像、穿行或飞越电影、或用于识别染色血管内的特定组织的颜色增强处理。这些3D图像可以在计算机上任意移动，旋转，移位或横截面切片。代表性静态
DECEMBER 2014: 1280–8 1283 or optical coherence tomography are in vogue. However, these techniques still have limitations in defining histological structure of the plaque, and advances in histology techniques might enhance the contribution of pathological studies. Mitsumori et al.(1), have developed a technique called “film tomography” to prepare tissue sections with an adhesive film. An automatic microtome can consecutively cut the paraffin-embedded tissue blocks into 6-μm-thick sections (approximately 1,600 slides from a 1-cm long tissue sample) and then put the sections on a long film roll in a row. The sections-onfilm are automatically placed in a plastic frame that can be processed with usual pathological or immunohistochemical stains.This is the first report of applying this technique to 3D assessment of atherosclerosis of postmortem human coronary arteries. Consecutive slides could be reconstructed as a longitudinal section, an exterior imaging of the whole vessel body, a translucent whole vessel imaging for depiction of tissue distribution, a walk-through or fly-through movie, or color enhancement processing for identifying specific tissue within the stained vessel. These 3D images can be moved, rotated, shifted, or cross sectionally sliced arbitrarily on the computer. Representative static