Endothelial Cell-derived Microparticles Loaded with Iron Oxide Nanoparticles: Feasibility of MR Imaging Monitoring in Mice
Endothelial Cell-derived Microparticles Loaded with Iron Oxide Nanoparticles: Feasibility of MR Imaging Monitoring in Mice
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DOI：
10.1148/radiol.11111329
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发表时间：
2012-04-01
期刊：
影响因子：
19.7
通讯作者：
Rautou, Pierre-Emmanuel
中科院分区：
文献类型：
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作者：
Al Faraj, Achraf;Gazeau, Florence;Rautou, Pierre-Emmanuel
目的：为了评估在内皮微粒（EMPs）中装载氧化铁纳米颗粒的可行性，从而使其非侵入性监测与磁共振（MR）imaging in mice.Materials and Methods：实验由法国农业部批准。首先用阴离子超顺磁性纳米颗粒标记的内皮细胞被刺激以产生EMP，在其内部隔室中携带纳米颗粒。C57 BL/6小鼠接受静脉内注射的纳米颗粒加载的EMP，游离的纳米颗粒，或纳米颗粒加载的EMP的上清液。使用4.7 T MR成像设备进行1周随访，使用梯度回波序列对脾、肝和肾进行成像，使用径向超短回波时间序列对肺进行成像。通过使用学生t检验进行比较。结果：在肝脏和脾脏中注射后5分钟内容易检测到由纳米颗粒加载的EMP或游离纳米颗粒诱导的信号强度损失，在脾脏中的磁性EMP具有更显著的效果。纳米粒子负载的电磁脉冲和自由的纳米粒子的信号强度衰减的动力学不同。在注射载有纳米颗粒的EMP的上清液的小鼠中没有观察到信号强度变化，证实细胞没有释放游离的纳米颗粒，而只是与EMP结合。结果证实，通过使用Perls染色和免疫荧光analysis.Conclusion：的战略，以产生电磁脉冲的磁特性允许非侵入性的MR成像评估和后续的电磁脉冲，并打开前景成像的影响，这些细胞载体的疾病。(C)RSNA，2012年
Purpose: To assess the feasibility of loading iron oxide nanoparticles in endothelial microparticles (EMPs), thereby enabling their noninvasive monitoring with magnetic resonance (MR) imaging in mice.Materials and Methods: Experiments were approved by the French Ministry of Agriculture. Endothelial cells, first labeled with anionic superparamagnetic nanoparticles, were stimulated to generate EMPs, carrying the nanoparticles in their inner compartment. C57BL/6 mice received an intravenous injection of nanoparticle-loaded EMPs, free nanoparticles, or the supernatant of nanoparticle-loaded EMPs. A 1-week follow-up was performed with a 4.7-T MR imaging device by using a gradient-echo sequence for imaging spleen, liver, and kidney and a radial very-short-echo time sequence for lung imaging. Comparisons were performed by using the Student t test.Results: The signal intensity loss induced by nanoparticle-loaded EMPs or free nanoparticles was readily detected within 5 minutes after injection in the liver and spleen, with a more pronounced effect in the spleen for the magnetic EMPs. The kinetics of signal intensity attenuation differed for nanoparticle-loaded EMPs and free nanoparticles. No signal intensity changes were observed in mice injected with the supernatant of nanoparticle-loaded EMPs, confirming that cells had not released free nanoparticles, but only in association with EMPs. The results were confirmed by using Perls staining and immunofluorescence analysis.Conclusion: The strategy to generate EMPs with magnetic properties allowed noninvasive MR imaging assessment and follow-up of EMPs and opens perspectives for imaging the implications of these cellular vectors in diseases. (C) RSNA, 2012