In vivo MR studies of glycine and glutathione metabolism in a rat mammary tumor.
In vivo MR studies of glycine and glutathione metabolism in a rat mammary tumor.
复制标题
DOI：
10.1002/nbm.1745
复制
发表时间：
2012-02
影响因子：
2.9
通讯作者：
Gamcsik, Michael P.
中科院分区：
文献类型：
--
作者：
Thelwall, Peter E.;Simpson, Nicholas E.;Rabbani, Zahid N.;Clark, M. Daniel;Pourdeyhimi, Roxana;Macdonald, Jeffrey M.;Blackband, Stephen J.;Gamcsik, Michael P.
通过磁共振成像和光谱学，在完整的 R3230Ac 大鼠肿瘤体内无创地监测甘氨酸向谷胱甘肽的代谢。通过跟踪静脉输注的[2-13C]-甘氨酸到谷胱甘肽甘氨酰残基中的同位素标记来跟踪代谢。通过非定域 13C 光谱检测来自 [2-13C]-甘氨酸和 γ-谷氨酰半胱氨酰-[2-13C]-甘氨酸（13C-谷胱甘肽）的信号，因为这些共振与背景信号不同。此外，利用光谱成像方法，观察到谷胱甘肽在体内肿瘤分布的异质性。体内光谱还检测到[2-13C]-甘氨酸的同位素掺入丝氨酸的2-和3-碳中。对肿瘤组织提取物的分析显示，通过丝氨酸羟甲基转移酶和甘氨酸裂解系统途径的代谢，将[2-13C]-甘氨酸的单个和多个标记掺入到丝氨酸中。提取物的质谱分析还表明，同位素标记的丝氨酸通过转硫途径进一步代谢，因为 13C 同位素标记出现在谷胱甘肽的甘氨酰基和半胱氨酰基残基中。我们的研究证明了使用磁共振成像和光谱学来监测氧化应激防御的核心肿瘤代谢过程。
The metabolism of glycine into glutathione was monitored noninvasively in vivo in intact R3230Ac rat tumors by magnetic resonance imaging and spectroscopy. Metabolism was tracked by following the isotope label from intravenously infused [2-13C]-glycine into the glycinyl residue of glutathione. Signals from [2-13C]-glycine and γ-glutamylcysteinyl-[2-13C]-glycine (13C-glutathione) were detected by nonlocalized 13C spectroscopy as these resonances are distinct from background signals. In addition, using spectroscopic imaging methods, heterogeneity in the in vivo tumor distribution of glutathione was observed. In vivo spectroscopy also detected isotope incorporation from [2-13C]-glycine into both the 2- and 3-carbons of serine. Analyses of tumor tissue extracts show single and multiple label incorporation from [2-13C]-glycine into serine from metabolism through the serine hydroxymethyltransferase and glycine cleavage system pathways. Mass spectrometric analysis of extracts also shows that isotope-labeled serine is further metabolized via the transsulfuration pathway as the 13C-isotope labels appear in both the glycinyl- and the cysteinyl-residue of glutathione. Our studies demonstrate the use of magnetic resonance imaging and spectroscopy for monitoring tumor metabolic processes central to oxidative stress defense.
登录
查看更多内容
影响因子：
11.2
作者：
Schroeder, T;Yuan, H;Dewhirst, MW
通讯作者：
Dewhirst, MW
影响因子：
29.4
作者：
Larsson, Susanna C.;Giovannucci, Edward;Wolk, Alicja
通讯作者：
Wolk, Alicja
影响因子：
2.8
作者：
BODENHAUSEN, G;RUBEN, DJ
通讯作者：
RUBEN, DJ
DOI：
10.1016/j.ijrobp.2004.02.020
发表时间：
2005-01-01
影响因子：
7
作者：
Hedley, DW;Nicklee, T;Hill, RP
通讯作者：
Hill, RP
DOI：
10.1016/s0360-3016(01)02716-x
发表时间：
2002-03-01
影响因子：
7
作者：
Vukovic, V;Nicklee, T;Hedley, DW
通讯作者：
Hedley, DW