Neural plasticity occurs in the adrenal medulla of asthmatic rats
Neural plasticity occurs in the adrenal medulla of asthmatic rats
复制标题
哮喘大鼠肾上腺髓质发生神经可塑性
DOI：
10.3760/cma.j.issn.0366-6999.2010.10.020
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发表时间：
2010-05-20
影响因子：
6.1
通讯作者：
Nie Hua-ping
中科院分区：
文献类型：
--
作者：
Feng Jun-tao;Li Xiao-zhao;Nie Hua-ping
背景哮喘的呼吸道症状与肾上腺素分泌减少有关，这可能归因于机体内神经生长因子(NGF)的升高。为了解哮喘大鼠肾上腺髓质神经内分泌的变化，将16只大鼠随机分为对照组和哮喘组，用卵白蛋白(OVA)致敏激发哮喘大鼠。采用酶联免疫吸附试验(EL ISA)检测大鼠血清NGF、肾上腺素和去甲肾上腺素水平，免疫组织化学方法检测肾上腺髓质NGF的表达，并用电子显微镜观察肾上腺髓质超微结构的变化。结果表明，与对照组相比，哮喘大鼠肾上腺素水平降低，而去甲肾上腺素水平无显著差异。哮喘大鼠肾上腺髓质内神经节细胞较对照组增多，NGF免疫组织化学染色主要定位于这些神经节细胞。电子显微镜下可见哮喘大鼠肾上腺髓质嗜铬颗粒密度降低，核膜缩小。结论哮喘大鼠肾上腺髓质的神经支配发生了改变，可能是导致肾上腺素减少的原因之一。中华医学杂志2010；123(10)：1333-1337
Background Airway symptoms in asthma are related to decrease of epinephrine secretion, which may be ascribed to elevated nerve growth factor (NGF) in the organism. The aim of this study was to monitor the neuroendocrine alteration in the adrenal medulla of asthmatic rats.Methods Sixteen rats were randomly divided into two groups (n=8), control group and asthma group, and the asthmatic rats were sensitized and challenged with ovalbumin (OVA). The levels of NGF, epinephrine and norepinephrine in serum were detected by enzyme linked immunosorbent assay (ELISA), the NGF expression in adrenal medulla was detected by immunohistochemistry, and the changes in the ultrastructure of the adrenal medulla was observed by electron microscopy.Results The NGF expression was increased in asthmatic rats compared with control rats. Compared with control rats, the results indicated that the epinephrine level was decreased in asthmatic rats, but no significant difference was found in norepinephrine levels. We found more ganglion cells in the adrenal medulla of asthmatic rats than in control rats, with NGF immunostaining mainly located in these ganglion cells. Electron microscopic images showed the density of chromaffin granula decreased and there was shrunken nucleolemma in the adrenal medullary cells of asthmatic rats.Conclusion The innervation of the adrenal medulla is changed in asthmatic rats, and it may contribute to the epinephrine decrease in asthma. Chin Med J 2010;123(10):1333-1337