R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein,, RGS9-1
R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein,, RGS9-1
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DOI：
10.1073/pnas.152094799
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发表时间：
2002-07-23
影响因子：
11.1
通讯作者：
Wensel, TG
中科院分区：
文献类型：
--
作者：
Hu, G;Wensel, TG
G蛋白信号调节因子（RGS）-9-1（.）在脊椎动物光感受器中，G（β 5）复合物形成G（β 5）的GT α加速蛋白。虽然复合物在体外表达时是可溶的，但从膜中提取内源性蛋白质需要洗涤剂。去污剂提取物含有RGS 9 -1、（G β）5、G（Glutamat）和25-kDa磷蛋白R9 AP（RGS 9 -1-锚蛋白）的复合物。R9 AP在人类和小鼠中都由一个无内含子基因编码。从小鼠、牛、人、斑马鱼和非洲爪蟾获得全部或部分cDNA或基因组克隆。R9 AP mRNA仅在视网膜中检测到，而蛋白质仅在光感受器中检测到。R9 AP结合RGS 9 -1的N-末端结构域，并通过C-末端跨膜螺旋将其锚定到盘膜。
The regulator of G protein signaling (RGS)-9-1(.)G(beta5) complex forms the GTPase accelerating protein for G(alphat) in vertebrate photoreceptors. Although the complex is soluble when expressed in vitro, extraction of the endogenous protein from membranes requires detergents. The detergent extracts contain a complex of RGS9-1, (Gbeta)5, G(alphat), and a 25-kDa phosphoprotein, R9AP (RGS9-1-Anchor Protein). R9AP is encoded by one intronless gene in both human and mouse. Full or partial cDNA or genomic clones were obtained from mice, cattle, human, zebrafish, and Xenopus laevis. R9AP mRNA was detected only in the retina, and the protein only in photoreceptors. R9AP binds to the N-terminal domain of RGS9-1, and anchors it to the disk membrane via a C-terminal transmembrane helix.