Transcriptome analysis ofPlantago majoras a phytoremediator to identify some genes related to cypermethrin detoxification
Transcriptome analysis ofPlantago majoras a phytoremediator to identify some genes related to cypermethrin detoxification
复制标题
车前子作为植物修复剂的转录组分析，以确定与氯氰菊酯解毒相关的一些基因
DOI：
10.1007/s11356-020-10774-4
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发表时间：
2020-09-21
影响因子：
5.8
通讯作者：
Hu, Zhaonong
中科院分区：
文献类型：
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作者：
Aioub, Ahmed A. A.;Zuo, Yayun;Hu, Zhaonong
氯氰菊酯（CYP）是一种有毒的人造化合物，属于拟除虫菊酯类杀虫剂，会污染环境。车前草（PM）具有生物量高、抗逆性强等诸多优异优点，能够提高植物修复的功效。到目前为止，还没有研究直接或间接对 CYP 胁迫下的 PM 进行转录组分析（RNA-seq）。本研究的目的是鉴定 PM 中与 CYP 解毒（10 μg mL-1）相关的基因，并与对照进行比较。本研究采用华大智造自主研发的BGISEQ-500高通量测序技术对大蠹转录组进行测序。构建了六个文库，包括（CK_1、CK_2 和 CK_3）和（CYP_1、CYP_2 和 CYP_3），对参与 CYP 解毒的转录本进行了测序。我们的数据显示，从头组装生成了 138,806 个 unigene，平均长度为 1129 bp。分析样本间 KEGG 数据库的注释结果，发现与对照相比，CYP 处理下有 37,177 个差异表达基因 (DEG)，其中 18,062 个下调，19,115 个上调。一组细胞色素 P450 (Cyt P450) 的 107 个基因、谷胱甘肽 S-转移酶 (GST) 的 43 个基因、糖基转移酶 (GT) 的 25 个基因、ABC 转运蛋白的 113 个基因、多药和毒素外排 (MATE) 的 21 个基因、寡肽转运蛋白 (OPT) 的 11 个基因和寡肽转运蛋白 (OPT) 的 3 个基因。金属硫蛋白（MT）显着上调。通过使用定量实时 PCR (qRT-PCR)，证实了 12 个随机选择的 DEG 的基因表达结果，显示了 PM 组织中对 CYP 的不同反应模式。此外，CYP胁迫下PM中Cyt P450和GST的酶活性在根和叶中均显着高于对照。本研究通过鉴定与植物修复相关的高表达基因，为了解植物修复中使用的植物的代谢途径提供了线索，从而可用于增强农药解毒并减少污染问题。
Cypermethrin (CYP) is a toxic manmade chemical compound belonging to pyrethroid insecticides contaminating the environment. Plantago major (PM) has numerous excellent advantages like high biomass yield and great stress tolerance, which make it able to increase the efficacy of phytoremediation. So far, no study has directly or indirectly made a transcriptome analysis (RNA-seq) of PM under CYP stress. The aim of this study is to identify the genes in PM related to CYP detoxification (10 μg mL-1) and compared with control. In this study, BGISEQ-500 high-throughput sequencing technology independently developed by BGI was used to sequence the transcriptome of P. major. Six libraries were constructed including (CK_1, CK_2, and CK_3) and (CYP_1, CYP_2, and CYP_3) were sequenced for transcripts involved in CYP detoxification. Our data showed that de novo assembly generated 138,806 unigenes with an average length of 1129 bp. Analyzing the annotation results of the KEGG database between the samples revealed 37,177 differentially expressed genes (DEGs), 18,062 down- and 19,115 upregulated under CYP treatment compared with control. A set of 107 genes of cytochrome P450 (Cyt P450), 43 genes of glutathione S-transferases (GST), 25 genes of glycosyltransferases (GTs), 113 genes from ABC transporters, 21 genes from multidrug and toxin efflux (MATE), 11 genes from oligopeptide transporter (OPT), and 3 genes of metallothioneins (MT) were upregulated notably. By using quantitative real-time PCR (qRT-PCR), the results of gene expression for 12 randomly selected DEGs were confirmed, showing the different patterns of response to CYP in PM tissues. Furthermore, the enzyme activity of Cyt P450 and GST in PM under CYP stress was significantly increased in roots and leaves than in control. This study introduces a clue to understand the metabolic pathways of plants used in phytoremediation by identifying the highly expressed genes related to phytoremediation which would be utilized to enhance pesticide detoxification and reduce pollution problem.