Different strains of mice present distinct lung tissue mechanics and extracellular matrix composition in a model of chronic allergic asthma
Different strains of mice present distinct lung tissue mechanics and extracellular matrix composition in a model of chronic allergic asthma
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DOI：
10.1016/j.resp.2008.12.003
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发表时间：
2009-02-28
影响因子：
2.3
通讯作者：
Rocco, Patricia R. M.
中科院分区：
文献类型：
--
作者：
Antunes, Mariana A.;Abreu, Soraia C.;Rocco, Patricia R. M.
遗传因素对哮喘的影响是众所周知的，但了解得很少。在慢性过敏性哮喘模型中，我们验证了不同品系的小鼠表现出不同的肺组织条力学的假设，并且这些力学差异可能与肺实质中细胞外基质成分和/或收缩元素的变化潜在地相关。对卵蛋白致敏和激发前后C57BL/10、BALB/c和A/J小鼠给予乙酰胆碱(ACh)前后的振荡机制进行了分析。在对照组中，A/J小鼠的组织弹性(E)和阻力(R)、胶原和弹性纤维含量以及α-肌动蛋白含量高于BALB/c小鼠，而后者又高于C57BL/10小鼠。无论小鼠品系如何，卵蛋白攻击的动物都观察到了类似的反应模式。与C57BL/10小鼠[E：9.4%，R：11%]相比，卵蛋白攻击后A/J[E：22%，R：18%]小鼠的E和R增加[E：22%，R：18%]。(C)2008爱思唯尔B.V.保留所有权利。
The impact of genetic factors on asthma is well recognized but poorly understood. We tested the hypothesis that different mouse strains present different lung tissue strip mechanics in a model of chronic allergic asthma and that these mechanical differences may be potentially related to changes of extracellular matrix composition and/or contractile elements in lung parenchyma. Oscillatory mechanics were analysed before and after acetylcholine (ACh) in C57BL/10, BALB/c, and A/J mice, subjected or not to ovalbumin sensitization and challenge. In controls, tissue elastance (E) and resistance (R), collagen and elastic fibres' content, and alpha-actin were higher in A/J compared to BALB/c mice, which, in turn, were more elevated than in C57BL/10. A similar response pattern was observed in ovalbumin-challenged animals irrespective of mouse strain. E and R augmented more in ovalbumin-challenged A/J [E: 22%, R: 18%] than C57BL/10 mice [E: 9.4%, R: 11 %] after ACh In conclusion, lung parenchyma remodelled differently yielding distinct in vitro mechanics according to mouse strain. (C) 2008 Elsevier B.V. All rights reserved.