Fluorosis: A new model and new insights
Fluorosis: A new model and new insights
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DOI：
10.1177/154405910508400910
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发表时间：
2005-09-01
影响因子：
7.6
通讯作者：
Payne-Ferreira, TL
中科院分区：
文献类型：
--
作者：
Bartlett, JD;Dwyer, SE;Payne-Ferreira, TL
氟化物是预防龋齿的有效药物。然而，过量氟暴露如何导致氟中毒的机制仍然不确定。斑马鱼（Danio rerio）在其一生中表现出周期性的牙齿更换，从而在易受氟化物暴露影响的发育阶段提供持续的牙齿接触。斑马鱼的牙齿不包含真正的釉质，但由坚硬的釉质表面组成。因此，我们想知道斑马鱼是否可以作为氟斑牙研究的模式生物。氟化物处理的牙齿的扫描电子显微镜表明，釉质是有坑的和粗糙的，和FTIR分析表明，牙齿也含有显着较高的有机物含量时，与未处理的对照组相比。此外，我们第一次证明，在牙齿发育中的一个重要的信号分子（Alk8）的表达减少可能有助于观察到的氟中毒表型，细胞凋亡增加也可能在氟中毒的机制中发挥作用。
Fluoride is an effective agent for the prevention of dental caries. However, the mechanism of how excessive fluoride exposure causes fluorosis remains uncertain. Zebrafish ( Danio rerio) exhibit periodic tooth replacement throughout their lives, thereby providing continuous access to teeth at developmental stages susceptible to fluoride exposure. Zebrafish teeth do not contain true enamel, but consist of a hard enameloid surface. Therefore, we asked whether zebrafish could be used as a model organism for the study of dental fluorosis. Scanning electron microscopy of fluoride-treated teeth demonstrated that the enameloid was pitted and rough, and FTIR analysis demonstrated that the teeth also contained a significantly higher organic content when compared with untreated controls. Furthermore, we demonstrate for the first time that decreased expression of an important signaling molecule (Alk8) in tooth development may contribute to the observed fluorotic phenotype, and that increased cell apoptosis may also play a role in the mechanism of fluorosis.