Abnormal microbiota composition in the ileocolonic mucosa of Crohn's disease patients as revealed by polymerase chain reaction-denaturing gradient gel electrophoresis
Abnormal microbiota composition in the ileocolonic mucosa of Crohn's disease patients as revealed by polymerase chain reaction-denaturing gradient gel electrophoresis
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DOI：
10.1097/01.mib.0000235828.09305.0c
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发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Garcia-Gil, L. Jesus
中科院分区：
文献类型：
--
作者：
Martinez-Medina, Margarita;Aldeguer, Xavier;Garcia-Gil, L. Jesus
背景资料：细菌可能在克罗恩病（CD）的发病机制中发挥作用，与正常健康受试者相比，患者具有不同类型和密度的肠道微生物群。因此，本研究的目的是比较粘附到CD患者与健康subjects.Methods的粘膜的微生物群：聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）的16 S rRNA基因片段被用来确定存在于新鲜活检样品中的优势细菌物种从15个健康和19 CD科目的粘膜。2例溃疡性结肠炎患者和1例缺血性结肠炎患者也被包括在内。结果：根据其分子指纹，将大多数CD标本（88.2%）与大多数健康/溃疡性结肠炎/缺血性结肠炎标本（82.3%）区分开来，个体被聚为2组。此外，CD组患者间的微生物群变异性大于健康/溃疡性结肠炎组（P = 0.000）。从PCR-DGGE条带中获得141个序列，分为58个不同的突变类型，其中8个是新的。BLAST分析显示，74.5%的序列与从未培养过的细菌的序列相似。在CD样本中，梭菌属Ruminococcus扭矩和大肠杆菌的患病率值显着较高，而粪杆菌更常见于健康标本。偶尔发现致病性γ-蛋白菌，只有在CD粘膜microbiot.Conclusions：微生物附着到CD患者的回结肠粘膜是从健康受试者的区别。我们推测，易患CD的个体调节肠道微生物组成的能力较低，这导致微生物种群不稳定。
Background: Bacteria might play a role in the pathogenesis of Crohn's disease (CD), and patients harbor a different type and density of gut microbiota compared with normal healthy subjects. Thus, the aim of this study was to compare the microbiota adhered to the mucosa of CD patients with that of healthy subjects.Methods: Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene fragments was used to identify the dominant bacterial species present in fresh biopsy samples obtained from the mucosa of 15 healthy and 19 CD subjects. Two patients suffering from ulcerative colitis and 1 suffering from ischemic colitis also were included.Results: Individuals were clustered in 2 groups according to their molecular fingerprint, which differentiated the majority of CD specimens (88.2%) from the majority of healthy/ulcerative colitis/ ischemic colitis specimens (82.3%). In addition, the patient-to-patient variability in microbiota was greater within the CD cluster than in the healthy/ulcerative colitis cluster (P = 0.000). One hundred forty-one sequences were obtained from the PCR-DGGE bands that were grouped into 58 different phylotypes, 8 of which were novel. BLAST analysis revealed that 74.5% of the sequences were similar to those of bacteria that have never been cultivated. In CD samples, prevalence values for Clostridium spp Ruminococcus torques and Escherichia coli were significantly higher, whereas Faecalibacterium was more frequently found in healthy specimens. Opportunistic pathogenic gamma-proteobacteria were found occasionally, only in CD mucosal microbiota.Conclusions: Microbiota attached to the ileocolonic mucosa of CD patients is distinguishable from that of healthy subjects. We postulate that individuals who are predisposed to CD are less able to regulate the microbial makeup of their intestines, which leads to an unstable microbial population.