Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy

Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy
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DOI：
10.1364/ao.49.003323
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发表时间：
2010-06-10
期刊：
APPLIED OPTICS
影响因子：
1.9
通讯作者：
Midorikawa, Katsumi
Midorikawa, Katsumi
中科院分区：
工程技术4区
文献类型：
--
作者：
Hashimoto, Hiroshi;Isobe, Keisuke;Midorikawa, Katsumi

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我们利用非线性傅立叶变换光谱法测量了各种荧光蛋白的双光子激发（TPE）光谱。通过使用光谱范围为700至1100 nm的超宽带激光脉冲，以高光谱分辨率测量了六种典型荧光蛋白（SeBFP、Sapphire、eGFP、eCFP、Venus、DsRed）的绝对TPE光谱。 (C) 2010 美国光学学会
We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution. (C) 2010 Optical Society of America