THE SELF-SPLICING INTRON IN THE NEUROSPORA APOCYTOCHROME-B GENE CONTAINS A LONG READING FRAME IN FRAME WITH THE UPSTREAM EXON
THE SELF-SPLICING INTRON IN THE NEUROSPORA APOCYTOCHROME-B GENE CONTAINS A LONG READING FRAME IN FRAME WITH THE UPSTREAM EXON
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DOI：
10.1093/nar/16.3.1125
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发表时间：
1988-02-11
影响因子：
14.9
通讯作者：
OLIVE, J
中科院分区：
文献类型：
--
作者：
COLLINS, RA;REYNOLDS, CA;OLIVE, J
我们已经确定了DNA序列的内含子1和侧翼外显子的线粒体脱辅基细胞色素B基因的脉孢菌实验室菌株74 A和自然分离北非。与以前的报道相比，我们发现该内含子包含一个951碱基的开放阅读框（ORF），与上游外显子的框架。推测的内含子编码的蛋白质在长度、计算的等电点以及碱性、酸性、极性和非极性氨基酸的比例方面类似于其它内含子ORF;然而，没有发现类似于成熟酶样ORF特征的“十肽”的氨基酸序列。再加上先前的发现，即该内含子能够在没有蛋白质的情况下在体外自我剪接，这里讨论的观察结果提出了这样的可能性，即其他具有长的、符合读框的ORF的内含子也可能能够在体外进行RNA催化的剪接。
We have determined the DNA sequence of intron 1 and flanking exons in the mitochondrial apocytochrome b gene of the Neurospora laboratory strain 74A and the natural isolate North Africa. In contrast to a previous report, we find that this intron contains an open reading frame (ORF) of 951 bases in frame with the upstream exon. The putative intron-encoded protein resembles those of other intron ORFs with respect to length, calculated isoelectric point, and proportion of basic, acidic, polar, and non-polar amino acids; however, no amino acid sequences resembling the "decapeptides" characteristic of maturase-like ORFs were found. Coupled with the previous finding that this intron is capable of self-splicing in vitro in the absence of proteins, the observations discussed here raise the possibility that other introns with long, in-frame ORFs may also be capable of RNA-catalyzed splicing in vitro.