Characterization of the phylogenetic diversity of five novel species belonging to the genus Bifidobacterium: Bifidobacterium castoris sp. nov., Bifidobacterium callimiconis sp. nov., Bifidobacterium goeldii sp. nov., Bifidobacterium samirii sp. nov. and Bifidobacterium dolichotidis sp. nov.
Characterization of the phylogenetic diversity of five novel species belonging to the genus Bifidobacterium: Bifidobacterium castoris sp. nov., Bifidobacterium callimiconis sp. nov., Bifidobacterium goeldii sp. nov., Bifidobacterium samirii sp. nov. and Bifidobacterium dolichotidis sp. nov.
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DOI：
10.1099/ijsem.0.003306
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发表时间：
2019-05-01
影响因子：
2.8
通讯作者：
Ventura, Marco
中科院分区：
文献类型：
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作者：
Duranti, Sabrina;Lugli, Gabriele Andrea;Ventura, Marco
从欧洲河狸(Castor Fibre)、高氏绒猴(Callimicogoeldii)、黑帽松鼠亚种(Saimirioliviensssubsp.)中分离到5株双歧杆菌，分别为2020B(T)、2028B(T)、2033B(T)、2034B(T)和2036B(T)。Peruviens)和巴塔哥尼亚Mara(Dolichotispatagum)。这些菌株均为革兰氏阳性，兼性厌氧，果糖6-磷酸磷酸酮醇酶阳性，不能运动，不产孢子。基于16S rRNA基因序列、多位点序列(包括hsp60、rpoB、dna J、dna G和clpC基因)和核心基因组的系统发育分析表明，双歧杆菌2020B(T)、2028B(T)、2033B(T)、2034B(T)和2036B(T)分别与双歧杆菌DSM 23969(T)、双歧双歧杆菌LMG 11041(T)、双歧双歧杆菌LMG 10510(T)、双歧杆菌LMG 11596(T)、双歧杆菌LMG 30297(T)、伊氏双歧杆菌L30187(T)和兰氏双歧杆菌MG 30126(T)有密切的亲缘关系。根据分离菌株的基因组序列并结合表型分析进行进一步的基因分型，清楚地表明这些菌株与迄今已知的双歧杆菌种的每一种模式菌株都不同。因此，双歧杆菌属(Bifidbacterium Castoris sp.)11月(2020B(T)=1MG 30937(T)=CCUG 72816(T))；11月(2028B(T)=1MG 30938(T)=CCUG 72814(T))；11月(2033B(T)=1MG 30940(T)=CCUG 72817(T))；11月(2034B(T)=1MG 30939(T)=CCUG 72815(T))；11月(2036B(T)=1MG 30941(T)=CCUG 72818(T))为双歧杆菌新种。
Five Bifidobacterium strains, i.e. 2020B(T), 2028B(T), 2033B(T), 2034B(T) and 2036B(T), were isolated from European beaver (Castor fiber), Goeldi's marmoset (Callimicogoeldii), black-capped squirrel monkey (Saimiriboliviensissubsp. peruviensis) and Patagonian mara (Dolichotispatagonum). All of these isolates were shown to be Gram-positive, facultative anaerobic, ofructose 6-phosphate phosphoketolase-positive, non-motile and non-sporulating. Phylogenetic analyses based on 16S rRNA gene sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2020B(T), 2028B(T), 2033B(T), 2034B(T) and 2036B(T) exhibit close phylogenetic relatedness to Bifidobacterium biavatii DSM 23969(T), Bifidobacterium bifidum LMG 11041(T), Bifidobacterium choerinum LMG 10510(T), Bifidobacterium gallicum LMG 11596(T), Bifidobacterium imperatoris LMG 30297(T), Bifidobacterium italicum LMG 30187(T) and Bifidobacterium vansinderenii LMG 30126(T) , respectively. Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species. Thus, Bifidobacterium castoris sp. nov. (2020B(T) =LMG 30937(T) =CCUG 72816(T)), Bifidobacterium callimiconis sp. nov. (2028B(T) =LMG 30938(T) =CCUG 72814(T)), Bifidobacterium samirii sp. nov. (2033B(T) =LMG 30940(T) =CCUG 72817(T)), Bifidobacterium goeldii sp. nov. (2034B(T) =LMG 30939(T) =CCUG 72815(T)) and Bifidobacterium dolichotidis sp. nov. (2036B(T) =LMG 30941(T) =CCUG 72818(T)) are proposed as novel Bifidobacterium species.