CYANOBACTERIA-CYANOPHAGE INTERACTIONS IN CONTINUOUS CULTURE
CYANOBACTERIA-CYANOPHAGE INTERACTIONS IN CONTINUOUS CULTURE
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DOI：
10.1111/j.1365-2672.1981.tb01273.x
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
STEWART, WDP
中科院分区：
文献类型：
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作者：
BARNET, YM;DAFT, MJ;STEWART, WDP
在含LPP-DUN1噬菌体和APH-1噬菌体的恒温箱中分别培养丝状伯氏平展线虫594和单细胞悬铃虫时，蓝藻和噬菌体数量开始出现一系列的相互振荡，其大小依次递减，直到在两个系统中建立一个蓝藻和病毒的平衡种群。随着互动的进行，主角的进化发生了。在实验结束时，大多数钉螺种群表现出对APH-1的抗性。分离到两种抗性菌株，分别命名为PR1和PR2。PR1对野生型LPP-DUN1具有抗性，但对突变噬菌体敏感；PR2不受该噬菌体的影响。突变型噬菌体和P.boryanum的出现顺序和相互作用解释了种群数量的初始波动以及顶峰种群中寄主和病原菌的共存。P.boryanum PR2的生长速度慢于野生型和PR1，在没有噬菌体的情况下与野生型和PR1竞争较差。文中还讨论了结果的生态学意义。
When the filamentous Plectonema boryanum 594 or the unicellular Aphanothece stagnina were grown in chemostats with phages LPP-DUN1 and Aph-1, respectively, an initial series of reciprocal oscillations in cyanobacterial and phage numbers occurred; their magnitudes decreased successively until an equilibrium population of cyanobacteria and virus was established in each system. Evolution of the protagonists occurred as the interactions proceeded. Most of the A. stagnina population appeared resistant to Aph-1 at the end of the experiment. Two types of resistant P. boryanum, designated PR1 and PR2, were isolated. PR1 was resistant to wild-type LPP-DUN1 but susceptible to a mutant phage; PR2 was unaffected by the phage. The sequence of appearance and interaction of mutant phage and P. boryanum accounted for the initial fluctuations in population numbers, and the coexistence of host and pathogen in the climax population. P. boryanum PR2 grew more slowly than wild-type or PR1 and competed poorly with them in the absence of phage. The ecological implications of the results are discussed.