Genetic analysis and genome mapping in Raphanus
Genetic analysis and genome mapping in Raphanus
复制标题
DOI：
10.1139/g03-026
复制
发表时间：
2003-06-01
期刊：
影响因子：
3.1
通讯作者：
Lydiate, DJ
中科院分区：
文献类型：
--
作者：
Bett, KE;Lydiate, DJ
根据栽培萝卜（Raphanus sativus）和野生萝卜（Raphanus raphanistrum）杂交的减数分裂，绘制了第一张萝卜基因组遗传图谱。该杂交种产生了54个体的BC种群和85个体的F-2种群。在这些群体中，共检测了236个标记位点，使用了一组144个信息性的芸苔RFLP探针，这些探针以前用于其他十字花科植物的遗传定位。来自BC1和F-2群体的遗传图谱是完全共线的，并被整合成一个健壮的Raphanus图谱。细胞学观察表明，红花与野红花杂交具有严格的二价配对关系。通过鉴定9个广泛连锁群和缺乏标记位点聚类，证实了沿每条染色体长度的生产性配对。事实上，与其他作物品种相比，标记位点和杂交的分布更具随机性。Raphanus的遗传标记和参考图谱对今后该作物的性状定位和标记辅助育种，以及Raphanus基因向芸苔属作物的基因组间转移具有重要的价值。还讨论了与拟南芥和芸苔属植物比较作图的未来效益。
The first genetic map of the Raphanus genome was developed based on meiosis in a hybrid between Raphanus sativus (cultivated radish) and Raphanus raphanistrum (wild radish). This hybrid was used to produce a BC, population of 54 individuals and an F-2 population of 85 individuals. A total of 236 marker loci were assayed in these populations using a set of 144 informative Brassica RFLP probes previously used for genetic mapping in other crucifer species. The genetic maps derived from the BC1 and F-2 populations were perfectly collinear and were integrated to produce a robust Raphanus map. Cytological observations demonstrated strict bivalent pairing in the R. sativus x R. raphanistrum hybrids. Productive pairing along the length of each chromosome was confirmed by the identification of nine extensive linkage groups and the lack of clustering of marker loci. Indeed, the distributions of both marker loci and crossovers was more random than those reported for other crop species. The genetic markers and the reference map of Raphanus will be of considerable value for future trait mapping and marker-assisted breeding in this crop, as well as in the intergenomic transfer of Raphanus genes into Brassica crops. The future benefits of comparative mapping with Arabidopsis and Brassica species are also discussed.