Smart wound dressing for infection monitoring and NIR-triggered antibacterial treatment
Smart wound dressing for infection monitoring and NIR-triggered antibacterial treatment
复制标题
用于感染监测和近红外触发抗菌治疗的智能伤口敷料
DOI：
10.1039/c9bm02060h
复制
发表时间：
2020
影响因子：
6.6
通讯作者：
Lie Ma
中科院分区：
文献类型：
--
作者：
Bianbian Qiao;Qian Pang;Peiqi Yuan;Yilun Luo;Lie Ma
伤口感染是临床上的主要挑战，极大地阻碍了伤口愈合过程。开发能够在早期阶段感知细菌感染并提供按需治疗的智能伤口敷料非常重要。在这项工作中，开发了一种智能水凝胶伤口敷料，能够通过细菌环境中菁3（Cy 3）和菁5（Cy 5）的pH响应荧光共振能量转移（FRET）跃迁来监测细菌感染，并通过近红外（NIR）提供按需感染治疗开发了光触发抗生素释放。通过物理交联聚乙烯醇（PVA）和紫外线（UV）可切割的聚前药（GS-Linker-MPEG）制备智能水凝胶，其中负载Cy 3和Cy 5修饰的二氧化硅纳米颗粒（SNP-Cy 3/Cy 5），并基于Cy 3和Cy 5之间的FRET效应作为pH响应性荧光探针来检测细菌感染。此外，将上转换纳米颗粒（UCNP）装载到水凝胶中以切割UV响应性GS-连接体-MPEG并实现GS在细菌环境中的NIR响应性释放。体外研究表明，该智能水凝胶具有良好的吸水性、力学性能和生物相容性，是其应用于伤口敷料的必要条件。此外，水凝胶在酸性缓冲液和细菌溶液中显示出明显的FRET转变。在用NIR光照射水凝胶后，UCNP能够将NIR光转化为UV光以触发GS从水凝胶释放用于抗菌处理。这项研究有望为伤口感染的自我报告和有效治疗提供新的策略。
Wound infection is a major challenge in the clinic that greatly hinders the wound healing process. It is highly important to develop smart wound dressings that can sense bacterial infection at early stages and provide on-demand treatment. In this work, a smart hydrogel-based wound dressing capable of monitoring bacterial infection via a pH-responsive fluorescence resonance energy transfer (FRET) transition of Cyanine3 (Cy3) and Cyanine5 (Cy5) in a bacterial environment and providing on-demand treatment of infection via near infrared (NIR) light-triggered antibiotic release was developed. The smart hydrogel was prepared by physical crosslinking of polyvinyl alcohol (PVA) and an ultraviolet (UV)-cleavable polyprodrug (GS-Linker-MPEG), in which Cy3 and Cy5-modified silica nanoparticles (SNP-Cy3/Cy5) were loaded and acted as a pH-responsive fluorescent probe to detect bacterial infection based on the FRET effect between Cy3 and Cy5. Also, up-conversion nanoparticles (UCNP) were loaded into the hydrogels to cleave the UV-responsive GS-Linker-MPEG and achieve NIR-responsive release of GS in the bacterial environment. The in vitro studies proved that the smart hydrogels present good water absorption ability and excellent mechanical properties as well as good biocompatibility, which are necessary for their application in wound dressings. Moreover, the hydrogels showed obvious FRET transitions in both acidic buffer and bacteria solution. Upon irradiating the hydrogels with NIR light, UCNP were able to convert NIR light to UV light to trigger the release of GS from the hydrogels for antibacterial treatment. This research is expected to provide a new strategy for self-reporting and effective treatment of wound infection.