Classical swine fever virus diversity and evolution
Classical swine fever virus diversity and evolution
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DOI：
10.1099/0022-1317-77-6-1311
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发表时间：
1996-06-01
影响因子：
3.8
通讯作者：
Paton, D
中科院分区：
文献类型：
--
作者：
Lowings, P;Ibata, G;Paton, D
通过分析经典猪瘟病毒（CSFV） E2 （gp55）和NS5B基因的核苷酸序列数据，以及先前发表的5'NCR数据，我们能够将115株猪瘟病毒分离株分为两大群、五个亚群和两个不同的分离株。通过对E2区序列数据的分析，可以进一步区分。将这三种基于测序的方法与单克隆抗体（MAb）分型和限制性内切酶（RE）定位进行比较。虽然MAb和RE方法都证实了之前的分类，但分辨率较差。我们通过对6年期间在单一地理区域观察到的病毒变异进行分析，估计了CSFV的大致进化率。将这一建议的比率应用于我们推断出的每个猪瘟亚群，使我们能够计算出每个亚群的大致分化日期。
By analysing the nucleotide sequence data generated from both the E2 (gp55) and the NS5B genes of classical swine fever virus (CSFV), in addition to previously published data from the 5'NCR, we were able to divide 115 CSFV isolates into two major groups, five subgroups and two disparate isolates. Further discrimination was possible by analysis of sequence data from the E2 region. The three sequencing based methods were compared to monoclonal antibody (MAb) typing and to limited restriction enzyme (RE) mapping. Although both MAb and RE methods confirmed the previous classification the resolution was inferior. We estimated an approximate evolution rate for CSFV from an analysis of the virus variation observed in a single geographical area over a 6 year period. Applying this proposed rate to each of our deduced CSFV subgroups enabled us to calculate the approximate dates of divergence for each subgroup.