CONTROL OF OSCILLATING GLYCOLYSIS OF YEAST BY STOCHASTIC, PERIODIC, AND STEADY SOURCE OF SUBSTRATE - MODEL AND EXPERIMENTAL STUDY
CONTROL OF OSCILLATING GLYCOLYSIS OF YEAST BY STOCHASTIC, PERIODIC, AND STEADY SOURCE OF SUBSTRATE - MODEL AND EXPERIMENTAL STUDY
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DOI：
10.1073/pnas.72.10.3829
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
HESS, B
中科院分区：
文献类型：
--
作者：
BOITEUX, A;GOLDBETER, A;HESS, B
在酵母提取物中，通过实验确定了由周期性底物源夹带糖酵解振荡的类型和范围。次谐波夹带证明了糖酵解振荡器的非线性性质。底物输入的随机变化产生不规则波形和稳定周期的持续振荡。结果与磷酸果糖激酶（EC 2.7.1.11; ATP：D-果糖-6-磷酸1-磷酸转移酶）的变构模型的预测一致，磷酸果糖激酶是负责糖酵解周期性操作的酶。在底物来源恒定的情况下，模型和实验之间的比较进一步表明，糖酵解系统的振荡动力学可以令人满意地描述由磷酸果糖激酶模型。
Type and range of entrainment of glycolytic oscillations by a periodic source of substrate are determined experimentally in yeast extracts. Subharmonic entrainment proves the nonlinear nature of the glycolytic oscillator Random variation of the substrate input yields sustained oscillations of irregular waveform and stable period. The results agree with the predictions of an allosteric model for phosphofructokinase (EC 2.7.1.11; ATP:D-fructose-6-phosphate 1-phosphotransferase), which is the enzyme responsible for periodic operation of glycolysis. A comparison between model and experiment in the case of a constant source of substrate further indicates that the oscillatory dynamics of the glycolytic system can satisfactorily be described by the phosphofructokinase model.