Peripheral CD4+ T cell subsets and antibody response in COVID-19 convalescent individuals
Peripheral CD4+ T cell subsets and antibody response in COVID-19 convalescent individuals
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DOI：
10.1172/jci141054
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发表时间：
2020-12-01
影响因子：
15.9
通讯作者：
Zhou, Pengcheng
中科院分区：
文献类型：
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作者：
Gong, Fang;Dai, Yaping;Zhou, Pengcheng
背景在了解导致全球大流行的2019冠状病毒病（COVID-19）的病理生理学方面取得了显著进展。然而，对于COVID-19中抗体应答的关键性CD 4（+）T细胞群体的了解甚少。在这项研究中，我们使用流式细胞术和磁化学发光酶抗体免疫分析法对13名COVID-19恢复期患者的外周血CD 4（+）T细胞进行了全面分析，这些患者被定义为确认无SARS-CoV-2 2 2 2周。这些数据与临床特征相关。我们观察到，相对于健康个体，恢复期患者显示出改变的外周血CD 4（+）T细胞谱。具体而言，与其他病毒感染一致，在COVID-19的恢复期患者中发现了与SARS-CoV-2靶向抗体相关的cTfh 1细胞。与健康个体和轻中度疾病患者相比，重度疾病患者Tern和Tfh-em细胞的频率较高，但Tcm、Tfh-cm、Tfr和Tnaive细胞的频率较低。有趣的是，在康复期患者中，较高频率的cTfh-em细胞与入院时记录的较低血氧水平相关。这些观察结果可能构成COVID-19长期影响CD 4（+）T细胞稳态的残余效应，并解释了在我们的严重COVID-19队列中发现的类别转换病毒特异性抗体产生个体的最高比例。我们的研究证明了COVID-19恢复期患者的CD 4（+）T细胞与抗体产生之间的密切联系。
BACKGROUND. Marked progress is achieved in understanding the physiopathology of coronavirus disease 2019 (COVID-19), which caused a global pandemic. However, the CD4(+) T cell population critical for antibody response in COVID-19 is poorly understood.METHODS. In this study, we provided a comprehensive analysis of peripheral CD4(+) T cells from 13 COVID-19 convalescent patients, defined as confirmed free of SARS-CoV-2 for 2 to 4 weeks, using flow cytometry and magnetic chemiluminescence enzyme antibody immunoassay. The data were correlated with clinical characteristics.RESULTS. We observed that, relative to healthy individuals, convalescent patients displayed an altered peripheral CD4(+) T cell spectrum. Specifically, consistent with other viral infections, cTfh1 cells associated with SARS-CoV-2-targeting antibodies were found in COVID-19 covalescent patients. Individuals with severe disease showed higher frequencies of Tern and Tfh-em cells but lower frequencies of Tcm, Tfh-cm, Tfr, and Tnaive cells, compared with healthy individuals and patients with mild and moderate disease. Interestingly, a higher frequency of cTfh-em cells correlated with a lower blood oxygen level, recorded at the time of admission, in convalescent patients. These observations might constitute residual effects by which COVID-19 can impact the homeostasis of CD4(+) T cells in the long-term and explain the highest ratio of class-switched virus-specific antibody producing individuals found in our severe COVID-19 cohort.CONCLUSION. Our study demonstrated a close connection between CD4(+) T cells and antibody production in COVID-19 convalescent patients.