Differential expression of Ped gene candidates in preimplantation mouse embryos.
Differential expression of Ped gene candidates in preimplantation mouse embryos.
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Ped 候选基因在植入前小鼠胚胎中的差异表达。
DOI：
10.1095/biolreprod59.4.941
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发表时间：
1998
影响因子：
3.6
通讯作者：
Warner,CM
中科院分区：
文献类型：
--
作者：
Wu,L;Exley,GE;Warner,CM
Ped（植入前胚胎发育）基因影响小鼠植入前胚胎发育率和随后的存活率。主要组织相容性复合体 Q 区的四个相似串联基因——Q6、Q7、Q8 和 Q9——被鉴定为 Pedgene 候选基因。在这项研究中，通过逆转录聚合酶链反应和单核苷酸引物延伸测定对这些基因在植入前发育过程中的表达进行了检查和定量，以研究它们对 Pedgene 表型的贡献。发现 Q7/Q9 基因对在植入前小鼠胚胎中转录，而 Q6/Q8 基因对的转录无法检测到。 Q7和Q9均在来自一种Ped快速菌株C57BL/6的胚胎中表达，而仅Q9基因在另一种Ped快速菌株B6.K2中表达。这些结果表明Q7和Q9基因在小鼠中都可以起到Ped基因的作用。有趣的是，Q7和Q9基因在植入前胚胎中的表达模式与在脾淋巴细胞中的表达模式相同。然而，Q6和Q8基因在脾淋巴细胞中表达，但在植入前胚胎中不表达。用干扰素γ(γ-IFN)处理小鼠植入前胚胎并没有诱导Q6/Q8基因的表达，但增强了Q7/Q9基因的表达。这种差异转录模式的机制目前正在研究中。
ThePed(preimplantation embryonic development) gene influences the rate of mouse preimplantation embryonic development and subsequent survival. Four similar tandem genes in the Q region of the major histocompatibility complex—Q6,Q7,Q8, andQ9—were identified asPedgene candidates. In this study, expression of these genes during preimplantation development was examined and quantitated by reverse transcription-polymerase chain reaction and single nucleotide primer extension assays in order to investigate their contribution to thePedgene phenotype. TheQ7/Q9gene pair was found to be transcribed in preimplantation mouse embryos, whereas transcription of theQ6/Q8gene pair was undetectable. BothQ7andQ9are expressed in embryos from onePed faststrain, C57BL/6, while only theQ9gene is expressed in anotherPed faststrain, B6.K2. These results suggest that both theQ7andQ9genes can function as thePedgene in the mouse. Interestingly, the expression pattern of theQ7andQ9genes in preimplantation embryos is the same as in splenic lymphocytes. However, theQ6andQ8genes are expressed in splenic lymphocytes but not in preimplantation embryos. Treatment of mouse preimplantation embryos with interferon gamma (γ-IFN) did not induce expression of theQ6/Q8genes but enhanced expression of theQ7/Q9genes. The mechanism of this differential transcription pattern is currently under investigation.