Circumventing the optical diffraction limit with customized speckles
Circumventing the optical diffraction limit with customized speckles
复制标题
DOI：
10.1364/optica.411007
复制
发表时间：
2021-02-20
期刊：
影响因子：
10.4
通讯作者：
Cao, Hui
中科院分区：
文献类型：
--
作者：
Bender, Nicholas;Sun, Mengyuan;Cao, Hui
散斑图案已广泛应用于重影成像、动态散斑照明显微镜、结构照明显微镜和光声波动成像等成像技术中。控制散斑统计特性的能力的最新进展使得能够针对特定成像应用定制散斑图案。在这项工作中，我们为基于荧光光开关的并行非线性图案照明显微镜设计和创建特殊的散斑图案。我们提出了一个原理验证实验演示，我们获得的空间分辨率比我们的装置中照明光学器件的衍射极限高三倍。此外，我们还表明，定制散斑的性能远远优于标准散斑。我们的工作表明，定制散斑是并行超分辨率显微镜中的有效工具。 (C) 2021 年美国光学协会根据 OSA 开放获取出版协议的条款
Speckle patterns have been used widely in imaging techniques such as ghost imaging, dynamic speckle illumination microscopy, structured illumination microscopy, and photoacoustic fluctuation imaging. Recent advances in the ability to control the statistical properties of speckles has enabled the customization of speckle patterns for specific imaging applications. In this work, we design and create special speckle patterns for parallelized nonlinear pattern-illumination microscopy based on fluorescence photoswitching. We present a proof-of-principle experimental demonstration where we obtain a spatial resolution three times higher than the diffraction limit of the illumination optics in our setup. Furthermore, we show that tailored speckles vastly outperform standard speckles. Our work establishes that customized speckles are a potent tool in parallelized super-resolution microscopy. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement