mir-127-3p inhibits the proliferation of myocytes by targeting KMT5a
mir-127-3p inhibits the proliferation of myocytes by targeting KMT5a
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mir-127-3p 通过靶向 KMT5a 抑制肌细胞增殖
DOI：
10.1016/j.bbrc.2018.06.104
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发表时间：
2018
影响因子：
3.1
通讯作者：
Mo Delin
中科院分区：
文献类型：
--
作者：
Yuan Renqiang;Zhang Xumeng;Fang Ying;Nie Yaping;Cai Shufang;Chen Yaosheng;Mo Delin
MicroRNA是一类高度保守的约20个核苷酸的非编码RNA，在转录后调节基因表达。在骨骼肌的发育过程中研究了许多miRNA，如miR-1、miR-206和miR-133。我们前期的研究发现miR-127- 3 p在猪胚胎骨骼肌中高表达，但其在肌肉发育中的具体功能尚不清楚。在本研究中，我们检测到miR-127- 3 p在成年小鼠的骨骼肌、心肌和增殖的C2 C12细胞系中也有高表达。过表达miR-127- 3 p对C2 C12细胞的分化几乎没有影响。而miR-127- 3 p对C2 C12细胞的增殖有明显的抑制作用。此外，我们确定KMT 5a作为靶基因，当引入miR-127- 3 p模拟物时，其在mRNA和蛋白水平上均下调。此外，在miR-127- 3 p处理的细胞中KMT 5a过表达挽救了miR-127- 3 p对C2 C12增殖的影响。总之，我们的数据表明，miR-127- 3 p通过KMT 5a调节心肌细胞的增殖。
MicroRNAs are a class of highly conserved ∼20 nucleotides non-coding RNAs that post-transcriptionally regulate gene expression. Many miRNAs were studied in the development of skeletal muscle, such as miR-1, miR-206, and miR-133. In our previous study, miR-127-3p was found highly expressed in porcine fetal skeletal muscle, whereas the detailed functions of miR-127-3p in muscle development is still unclear. In this study, we detected that miR-127-3p also highly expressed in skeletal muscle, cardiac muscle of adult mice and proliferative C2C12 cell lines. Overexpression of miR-127-3p almost has no effects on differentiation of C2C12 cell lines. However, miR-127-3p significantly inhibited the cell proliferation of C2C12 cells. Moreover, we identified KMT5a as a target gene that was down-regulated in both mRNA and protein level when miR-127-3p mimics were introduced. Furthermore, KMT5a overexpression in miR-127-3p treated cells rescued the influence of miR-127-3p on C2C12 proliferation. In brief, our data reveals that miR-127-3p regulates the proliferation of myocytes through KMT5a.