Conservation of fruit dehiscence pathways between Lepidium campestre and Arabidopsis thaliana sheds light on the regulation of INDEHISCENT
Conservation of fruit dehiscence pathways between Lepidium campestre and Arabidopsis thaliana sheds light on the regulation of INDEHISCENT
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DOI：
10.1111/tpj.12321
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发表时间：
2013-11-01
期刊：
影响因子：
7.2
通讯作者：
Theissen, Guenter
中科院分区：
文献类型：
--
作者：
Lenser, Teresa;Theissen, Guenter
果实开放方式是一个重要的农艺性状和进化性状，已在主要植物模式系统拟南芥中得到深入研究。由于果实形态在物种之间变化很大，并且在育种过程中也经常是人工选择的目标，因此研究果实形态的变化是否可能改变导致果实开放的发育途径是有趣的。在这里，我们已经研究了在独行菜，一个菊科物种，形成短角果，而不是长角果的果实发育。发现具有拟南芥果实发育基因（ALCATRAZ、FRUITFULL、INDEHISCENT和SHATTERPROOF 1，2）的直系同源物的表达水平改变的转基因L.campestre植物在果实开裂方面有缺陷，并且解剖切片揭示了与在相应的拟南芥突变体中发现的组织图案相似的变化。基因表达分析表明，在基因调控电路的高度保守性，表明，尽管果实形态差异很大，果实开放的过程中保持基本不变的物种之间。有趣的是，我们的数据确定ALCATRAZ作为一个负调节INDEHISCENT在L. campestre。通过突变体分析，我们在拟南芥中也发现了相同的调控关系，从而为ALCATRAZ如何驱动分离层的形成提供了新的线索。
The mode of fruit opening is an important agronomic and evolutionary trait that has been studied intensively in the major plant model system Arabidopsis thaliana. Because fruit morphology is highly variable between species, and is also often the target of artificial selection during breeding, it is interesting to investigate whether a change in fruit morphology may alter the developmental pathway leading to fruit opening. Here we have studied fruit development in Lepidium campestre, a Brassicaceae species that forms silicles instead of siliques. Transgenic L.campestre plants with altered expression levels of orthologs of A.thaliana fruit developmental genes (ALCATRAZ,FRUITFULL,INDEHISCENT and SHATTERPROOF1,2) were found to be defective in fruit dehiscence, and anatomical sections revealed similar changes in tissue patterning as found in respective A.thaliana mutants. Gene expression analyses demonstrated a high degree of conservation in gene regulatory circuits, indicating that, despite great differences in fruit morphology, the process of fruit opening remains basically unchanged between species. Interestingly, our data identify ALCATRAZ as a negative regulator of INDEHISCENT in L.campestre. By mutant analysis, we found the same regulatory relationship in A.thaliana also, thereby shedding new light on how ALCATRAZ drives separation layer formation.