Gangliosides reduce leakage of aqueous-space markers from liposomes in the presence of human plasma.
Gangliosides reduce leakage of aqueous-space markers from liposomes in the presence of human plasma.
复制标题
在人血浆存在的情况下，神经节苷脂可减少脂质体中水空间标记物的渗漏。
DOI：
10.1016/0005-2736(85)90571-1
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Papahadjopoulos,D
中科院分区：
文献类型：
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作者：
Allen,TM;Ryan,JL;Papahadjopoulos,D
我们已经研究了糖脂在人血浆存在下减少水空间标记物从主要由卵磷脂组成的脂质体中泄漏的作用。脂质体为小单层（SUV）或大单层（LUV）。在胆固醇存在或不存在的情况下，以不同的摩尔比将单-，二-或三唾液酸神经节苷脂（GM，GD，GT）掺入脂质体双层中，检查脂质体内容物的泄漏。脂质体的渗漏随着神经节苷脂唾液酸的增加而减少。去唾液酸神经节苷脂对血浆存在下的钙黄绿素渗漏没有影响。神经节苷脂和胆固醇的稳定作用是协同的，并且含有10 mol%GT和33 mol%胆固醇的SUV在37°C下具有接近24小时的血浆中钙黄绿素渗漏的半衰期。LUV在血浆的存在下保留其内容物的时间比SUV，和神经节苷脂有一个额外的稳定作用。磷脂酰丝氨酸和硫苷脂也能够取代神经节苷脂稳定脂质体血浆诱导的泄漏。似乎神经节苷脂至少部分地通过其赋予表面负电荷的能力来稳定血浆中的脂质体。
We have studied the role of glycolipids in reducing leakage of aqueous-space markers from liposomes, composed primarily of egg phosphatidylcholine, in the presence of human plasma. Liposomes were either small unilamellar (SUV) or large unilamellar (LUV). Leakage of liposome contents as affected by the incorporation into the liposomal bilayer of mono-, di-, or trisialogangliosides (GM, GD, GT) at different molar ratios in the presence or absence of cholesterol was examined. Leakage from liposomes decreased with increasing ganglioside sialic acid. Asialogangliosides had no effect on calcein leakage in the presence of plasma. The stabilizing effect of gangliosides and cholesterol was synergistic, and SUV containing 10 mol% GTand 33 mol% cholesterol had a half-life for leakage of calcein in plasma at 37°C approaching 24 hours. LUV in the presence of plasma retained their contents longer than SUV, and gangliosides had an additional stabilizing effect. Phosphatidylserine and sulfatides were also capable of substituting for gangliosides in stabilizing liposomes to plasma-induced leakage. It appears that gangliosides stabilize liposomes in plasma at least in part through their ability to impart surface negative charge.