Small circular deoxyribonucleic acid of Drosophila melanogaster: homologous transcripts in the nucleus and cytoplasm.
Small circular deoxyribonucleic acid of Drosophila melanogaster: homologous transcripts in the nucleus and cytoplasm.
复制标题
果蝇的小环状脱氧核糖核酸：细胞核和细胞质中的同源转录物。
DOI：
10.1021/bi00557a036
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
Lengyel,JA
中科院分区：
文献类型：
--
作者：
Stanfield,SW;Lengyel,JA
DNA. We show here that transcripts homologousto small circular DNA are present in various RNA fractions. Nuclear poly (A+), nuclear poly (A-), and polysomal poly (A+) RNA drive 10, 7, and 20%, respectively, of in vitro labeled small circular DNA tracer into hybrid. Sequences complementary to smallcircular DNA are at least 10-fold more concentrated in nuclear poly (A+) RNA than in nuclear poly (A-) or po-Heterogeneous small circular DNA is an intriguing class of molecules for which a number of genetic regulatory func-tions has been proposed. This type of DNA has been described in a wide variety of eukaryotic organisms, including Neurospora, Euglena, trypanosomes, yeast, tobacco, Xenopus, chickens, and boar, and in cell culture lines from monkey, mouse, and humans (Agsteribbe et al., 1972; Nass & Ben-Shaul, 1972; Ono et al., 1971; Billheimer & Avers, 1969; Wong & Wildman, 1972; Delap & Rush, 1970; Buongior-no-Nardelli et al., 1976; Hotta & Bassel, 1965; Smith & Vinograd, 1972; Delap et al., 1978). We have previously described a number of properties of small circularDNA fromcultured cells of Drosophila melanogaster (Stanfield & Helinski, 1976; Stanfield & Lengyel, 1979). This DNA, isolated as covalently closed circular molecules, is heterogeneous in contour length, with an average size of 3300 NTP. 1* It is localized predominantly, if not entirely, in the nucleus and has a buoyant density indistinguishable from that of the main band of nuclear DNA. The sequence complexity of the major component (82%) of the small circular DNA is 1.8 X 104 NTP. A majority (possibly all) of the small circular DNA is homologous to middle rep-etitive sequences of Drosophila chromosomal DNA. Thermal
