Correlation between Ureaplasma spp. sub-group 1 and preterm pre-labour rupture of membranes revealed by an eMLST scheme
Correlation between Ureaplasma spp. sub-group 1 and preterm pre-labour rupture of membranes revealed by an eMLST scheme
复制标题
解脲支原体之间的相关性。
DOI：
10.1016/j.meegid.2018.12.025
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Zhang Jun
中科院分区：
文献类型：
--
作者：
Kong Yingying;Yang Tingting;Yang Ting;Ruan Zhi;Song Tiejun;Ding Honghui;Xie Xinyou;Zhang Jun
解脲支原体正被公认为与早产(PTB)和早产前胎膜破裂(PPROM)有关的重要病原体。本研究的目的是研究这种微生物在患有肺结核或产前胎膜破裂(PROM)或PPROM的母亲/新生儿对中的克隆性，以及亚型与PPROM的关系。总体而言，在93对诊断为肺结核、胎膜早破或胎膜早破的母亲/新生儿中，有50对被鉴定为解脲支原体。定植于羊水、脐带或胎盘。104例临床解脲支原体全部检出。样本(分别来自羊水、脐带和胎盘培养的50、30和24个)被纳入使用eMLST方案进行遗传谱系分析。共获得34条EST序列，其中优势EST16和EST41两条。有趣的是，六对母亲/新生儿在上述三个样本来源中显示出最大的差异。此外，系统发育分析显示有两个具有遗传意义的远端聚类，而聚类I包含了最多的临床菌株。有趣的是，在胎膜早破和胎膜早破的妇女中，第二类亚组1的患病率有显著差异。综上所述，在母婴配对中，第I群的分布明显高于第II群。此外，亚组1易通过特定的流行克隆谱系关联PPROM。
Ureaplasma spp. is gaining recognition as an important pathogen associated with preterm birth (PTB) and preterm pre-labour rupture of membranes (PPROM). The aim of this study was to investigate the clonality of this organism in maternal/neonatal pairs with PTB or pre-labour rupture of membranes (PROM) or PPROM and the association between sub-groups and PPROM. In total, 50 of 93 maternal/neonatal pairs that were diagnosed with PTB, PROM or PPROM were identified with Ureaplasma spp. colonized in the amniotic fluid or umbilical cord or placenta. All 104 clinicalUreaplasmaspp. samples (50, 30, and 24 cultured from amniotic fluid, umbilical cord, and placenta, respectively) were included for analysis of the genetic lineages using the eMLST scheme. A total of 34 eSTs were revealed, with two predominant eSTs (eST16 and eST41). Interestingly, six maternal/neonatal pairs displayed eST differences in the above three specimen sources. In addition, phylogenetic analysis showed two genetically significant distant clusters, and cluster I included the most clinical strains. Interestingly, there was a significant difference in the prevalence of sub-group 1 of cluster II between women with PPROM and those with PROM. In conclusion, the distribution of cluster I was predominately higher than that of cluster II in maternal/neonatal pairs. In addition, sub-group 1 was prone to associated PPROM through the specific epidemic clonal lineages.