Cortical visual functions can be preserved by subretinal RPE cell grafting in RCS rats
Cortical visual functions can be preserved by subretinal RPE cell grafting in RCS rats
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DOI：
10.1016/s0042-6989(03)00276-1
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发表时间：
2003-08-01
期刊：
影响因子：
1.8
通讯作者：
Lund, RD
中科院分区：
文献类型：
--
作者：
Girman, SV;Wang, S;Lund, RD
在皇家外科学院（RCS）大鼠的光感受器丧失在主要光感受器丧失发生之前，通过将视网膜色素上皮细胞系移植到视网膜下空间，限制了光感受器的丧失。本研究的目的是检查这种移植在多大程度上挽救了视觉皮质功能，以及挽救的程度如何与上丘记录的阈值反应相关。为了达到这个目的，在7个月大的时候，在这些动物的皮层通常对特定的视觉刺激没有反应的十分之一的时候，从皮层的核上层V1记录了单个单位的反应。最好的动物给出的皮质反应与正常情况相差无几。对于整个研究小组来说，在每个细胞测量的8个参数中，只有3个明显低于正常的非营养不良大鼠。总的来说，在更多的光感受器恢复的情况下，皮层中获得更好的单单位反应，这与更好的阈值反应相关。这些结果表明，可以通过视网膜下移植细胞系来保留离散的中央视觉反应，从而限制与进行性光感受器丧失相关的输入信号的慢性损失。2003爱思唯尔科学有限公司版权所有。
Photoreceptor loss in the Royal College of Surgeons (RCS) rat is limited by transplantation of retinal pigment epithelial cell lines into the subretinal space prior to the onset of major photoreceptor loss. The purpose of this study was to examine to what extent visual cortical function was rescued by such transplantation and how the degree of rescue correlated with threshold responses recorded in the superior colliculus. To achieve this, single unit responses were recorded from the supragranular layers of cortical area, V1, at 7 months of age at a tithe when the cortex in these animals is normally non-responsive to specific visual stimulation. The best animals gave cortical responses that were very little different from normal. For the whole group studied, of the eight parameters measured for each cell, only three were significantly less well tuned than in normal non-dystrophic rats. In general, better single unit responses in the cortex were obtained with more photoreceptor rescue and this correlated with better threshold responses.These results indicate that discrete central visual responses can be preserved by subretinal transplantation of a cell line which limits chronic loss of input signal associated with progressive photoreceptor loss. (C) 2003 Elsevier Science Ltd. All rights reserved.