CLONED VIRAL PROTEIN VACCINE FOR FOOT-AND-MOUTH-DISEASE - RESPONSES IN CATTLE AND SWINE
CLONED VIRAL PROTEIN VACCINE FOR FOOT-AND-MOUTH-DISEASE - RESPONSES IN CATTLE AND SWINE
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DOI：
10.1126/science.6272395
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发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
BACHRACH, HL
中科院分区：
文献类型：
--
作者：
KLEID, DG;YANSURA, D;BACHRACH, HL
编码口蹄疫 [FMD] 病毒 A12 免疫原性衣壳蛋白 VP3 的 DNA 序列（从病毒粒子 RNA 中制备）连接到设计用于表达来自大肠杆菌色氨酸启动子操纵子系统的嵌合蛋白的质粒。当用该质粒转化的大肠杆菌在色氨酸耗尽的培养基中生长时，.apprx。细胞总蛋白的 17% 是不溶且稳定的嵌合蛋白。纯化的嵌合蛋白在摩尔基础上与 VP3 平等竞争针对 FMD 病毒的特异性抗体。当将这种蛋白质接种到 6 头牛和 2 头猪体内时，会引发高水平的中和抗体，并针对 FMD 病毒的攻击提供保护。
A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease [FMD] virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When E. coli transformed with this plasmid was grown in tryptophan-depleted media, .apprx. 17% of the total cellular protein was an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to FMD virus. When inoculated into 6 cattle and 2 swine, this protein elicited high levels of neutralizing antibody and protection against challenge with FMD virus.