Centrifugal extraction of plasma from whole blood on a rotating disk
Centrifugal extraction of plasma from whole blood on a rotating disk
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DOI：
10.1039/b604145k
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发表时间：
2006-06-01
期刊：
影响因子：
6.1
通讯作者：
Ducree, Jens
中科院分区：
文献类型：
--
作者：
Haeberle, Stefan;Brenner, Thilo;Ducree, Jens
我们提出了一个离心过程中提取血浆从沉积物的滗析结构，终止计量血浆，这是很容易获得的后续磁盘上的处理。我们的技术仅在20秒内以40 Hz的中等旋转频率从5 μ l全血中提供2 μ l血浆。纯化血浆中的残留细胞浓度小于0.11%，与旋转频率无关。毛细管将提取的血浆连接到后续的盘上处理单元。
We present a centrifugal process for the extraction of plasma from sediment by a decanting structure, terminating with metered plasma which is readily available for subsequent on-disk processing. Our technique supplies 2 mu l plasma from 5 mu l of whole blood at moderate spinning frequencies of 40 Hz within 20 s, only. The residual cell concentration in the purified plasma amounts to less than 0.11 %, independent of the frequency of rotation. A capillary duct connects the extracted plasma to subsequent on-disk processing units.