Circulating calcitonin gene-related peptide and its placental origins in normotensive and preeclamptic pregnancies
Circulating calcitonin gene-related peptide and its placental origins in normotensive and preeclamptic pregnancies
复制标题
DOI：
10.1016/j.ajog.2006.04.006
复制
发表时间：
2006-12-01
影响因子：
9.8
通讯作者：
Yallampalli, Chandra
中科院分区：
文献类型：
--
作者：
Dong, Yuan-Lin;Chauhan, Madhu;Yallampalli, Chandra
目的：本研究旨在测定正常血压和子痫前期妊娠中母体和胎儿血浆降钙素基因相关肽的浓度，并探讨胎盘是否为其来源之一。研究设计：采集血压正常且伴有先兆子痫的孕妇的母血、脐带血和绒毛组织。用放射免疫法测定降钙素基因相关肽浓度。采用原位杂交和免疫组织化学方法检测胎盘绒毛降钙素基因相关肽信使核糖核酸的细胞定位和蛋白表达。结果：(1)孕妇血浆降钙素基因相关肽浓度随妊娠晚期升高，分娩后下降；(2)产妇和脐带血浆降钙素基因相关肽浓度与婴儿出生体重呈正相关；(3)与正常妊娠相比，子痫前期妊娠产妇血浆和脐带血浆降钙素基因相关肽水平均下降；(4)正常妊娠时，脐静脉血浆降钙素基因相关肽高于脐动脉，但子痫前期静脉与动脉无显著差异；(5)降钙素基因相关肽信使核糖核酸和蛋白在正常妊娠时由合胞滋养细胞和绒毛血管内皮细胞表达，但在子痫前期胎盘中仅见弱或未见染色；(6)外植体培养中，绒毛组织分泌降钙素基因相关肽具有时间依赖性，而子痫前期患者的绒毛组织产生的降钙素基因相关肽较少。结论：降钙素基因相关肽可能在母体血流动力学适应和胎儿生长发育中发挥潜在作用。降低循环降钙素基因相关肽水平可能参与先兆子痫的母胎病理生理。胎盘绒毛组织可能是妊娠期降钙素基因相关肽的潜在来源之一。(c) 2006 Mosby, Inc。版权所有。
Objective: The present study was designed to determine plasma calcitonin gene-related peptide concentration in both maternal and fetal circulations in normotensive and pre-eclamptic pregnancies and investigate whether placenta is 1 of its origins.Study design: Maternal blood, cord blood, and villous tissue were collected from women in normotensive pregnancies and complicated with pre-eclampsia. Calcitonin gene-related peptide concentrations were determined by radioimmunoassay. Cellular localizations of calcitonin gene-related peptide messenger ribonucleic acid and protein expressions in placental villi were determined by in situ hybridization and immunohistochemistry.Results: The following results were reached: (1) maternal plasma calcitonin gene-related peptide concentrations increased with advancing gestation but fell after delivery; (2) both maternal and cord plasma calcitonin gene-related peptide concentrations were positively correlated with the infant birth weights; (3) compared with normotensive pregnancies, calcitonin gene-related peptide levels in both maternal and cord plasma decreased in pregnancies with pre-eclampsia; (4) in normotensive pregnancies, the plasma calcitonin gene-related peptide of the umbilical vein was higher than the umbilical artery, but no significant differences between vein and artery in pre-eclampsia; (5) calcitonin gene-related peptide messenger ribonucleic acid and protein were expressed by syncytiotrophoblast cells and villous vascular endothelial cells in normotensive pregnancies, but only weak or absent staining was observed in pre-eclamptic placentas; and (6) calcitonin gene-related peptide is secreted by villous tissue in explant culture in a time-dependent manner, but less calcitonin gene-related peptide was produced by villous tissues from patients with pre-eclampsia.Conclusion: Calcitonin gene-related peptide may play potential roles in maternal hemodynamic adaptation and fetal growth. Decreased circulating calcitonin gene-related peptide levels may be involved in maternal-fetal pathophysiology of pre-eclampsia. It is novel that placenta villous tissues might be one of the potential sources of calcitonin gene-related peptide during pregnancy. (c) 2006 Mosby, Inc. All rights reserved.