DNA end labeling (TUNEL) in Huntington's disease and other neuropathological conditions.
DNA end labeling (TUNEL) in Huntington's disease and other neuropathological conditions.
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DNA 末端标记 (TUNEL) 在亨廷顿舞蹈病和其他神经病理学疾病中的应用。
DOI：
10.1006/exnr.1995.1029
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Steindler,DA
中科院分区：
文献类型：
--
作者：
Thomas,LB;Gates,DJ;Richfield,EK;O'Brien,TF;Schweitzer,JB;Steindler,DA
Deoxyribonucleic acid of cells undergoing apoptosis is cleaved by a calcium-dependent endonuclease into oligonucleosomal-sized fragments. These fragments can be labeled using the enzyme terminal deoxynucleotidyl transferase so that the cells can be visualized immunohistochemically. Few investigators have evaluated this method in disease processes of the human central nervous system. The Tdt-mediated dUTP-biotin nick end labeling (TUNEL) technique has been investigated in preliminary studies of a variety of pathologic conditions of the human brain (e.g., gliomas, traumatic brain injury, Parkinson's disease, Parkinson's-Alzheimer's complex, multisystem atrophy, striatonigral degeneration). We focus, however, on Huntington's disease (HD) because of the availability of well-characterized pathological stages for study, and also because of the neurodegenerative diseases studied to date, only Huntington's disease revealed significant and consistent labeling with this method. This implies a possibly unique nature to the mechanism of cell death in Huntington's disease compared to the other neurodegenerative diseases studied. TUNEL+ neurons were found in Grade 1-4 HD neostriatum, while labeled astrocytes were found predominantly in the Grade 1 and 2 cases studied to date. TUNEL+ cells were also found in glioblastoma multiforme and traumatic brain injury. We conclude that while there appear to be several limitations associated with this technique, it may be useful for identifying both apoptosis and necrosis in certain neuropathological conditions.
