Temporal progressive antigen expression in radial glia after contusive spinal cord injury in adult rats
Temporal progressive antigen expression in radial glia after contusive spinal cord injury in adult rats
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DOI：
10.1002/glia.10203
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发表时间：
2003-04
期刊：
影响因子：
6.2
通讯作者：
S. Shibuya;O. Miyamoto;T. Itano;S. Mori;H. Norimatsu
中科院分区：
文献类型：
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作者：
S. Shibuya;O. Miyamoto;T. Itano;S. Mori;H. Norimatsu
在中枢神经系统的发育过程中，放射状胶质细胞是最早起源于神经上皮细胞的细胞之一。最近的研究报道，放射状胶质细胞具有神经干细胞的特性。我们分析了脊髓损伤(SCI)后神经胶质细胞的抗原表达和分布。SD大鼠于T11~12椎板切除，30g加力10min造成脊髓挫伤。损伤组大鼠分别于伤后24 h、1、4、12周取材。冰冻切片厚度为20-μm，分别位于损伤中心前后部5 mm和10 mm处。用3CB2(放射状胶质细胞的特异性标志物)、巢蛋白和胶质纤维酸性蛋白(GFAP)的抗体进行免疫组织化学染色。伤后1周，抗3CB2单抗结合的放射状胶质细胞已从软膜表面以下遍及整个白质。从伤后4周开始，3CB2在中央管周围灰质也有表达，以室管膜细胞和血管周围表达最强。在3CB2和GFAP或3CB2和Nestin的双重免疫组织化学染色中，可观察到在延伸至白质的树枝状突起的软膜下结构和灰质中的血管周围有共同表达。本研究证实了成年哺乳动物脊髓损伤后放射状胶质细胞的出现。软膜下星形胶质细胞来源的放射状胶质细胞可能在脊髓损伤后的神经修复和再生中发挥重要作用。Glia 42：172-183,2003。©2003 Wiley-Liss公司
In the development of the CNS, radial glial cells are among the first cells derived from neuroepithelial cells. Recent studies have reported that radial glia possess properties of neural stem cells. We analyzed the antigen expression and distribution of radial glia after spinal cord injury (SCI). Sprague‐Dawley rats had a laminectomy at Th11‐12, and spinal cord contusion was created by compression with 30g of force for 10 min. In the injury group, rats were examined at 24 h and 1, 4, and 12 weeks after injury. Frozen sections of 20‐μm thickness were prepared from regions 5 and 10 mm rostral and caudal to the injury epicenter. Immunohistochemical staining was performed using antibodies to 3CB2 (a specific marker for radial glia), nestin, and glial fibrillary acidic protein (GFAP). At 1 week after injury, radial glia that bound anti‐3CB2 MAb had spread throughout the white matter from below the pial surface. From 4 weeks after injury, 3CB2 expression was also observed in the gray matter around the central canal, and was especially strong around the ependymal cells and around blood vessels. In double‐immunohistochemical assays for 3CB2 and GFAP or 3CB2 and nestin, coexpression was observed in subpial structures that extended into the white matter as arborizing processes and around blood vessels in the gray matter. The present study demonstrated the emergence of radial glia after SCI in adult mammals. Radial glia derived from subpial astrocytes most likely play an important role in neural repair and regeneration after SCI. GLIA 42:172–183, 2003. © 2003 Wiley‐Liss, Inc.