A New Nanogold-Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG
A New Nanogold-Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG
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DOI：
10.1002/cjoc.200790238
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发表时间：
2007-09
影响因子：
5.4
通讯作者：
Zhiliang Jiang;Yan Li;Shuang-jiao Sun;Bing Chen
中科院分区：
文献类型：
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作者：
Zhiliang Jiang;Yan Li;Shuang-jiao Sun;Bing Chen
以柠檬酸三钠为原料制备了一种9 nm的金纳米粒子，并将其用于标记羊抗人IgG，制备了IgG免疫共振散射光谱探针。在pH5.8的缓冲溶液中，在聚乙二醇（PEG）存在下，金标羊抗人IgG与IgG发生免疫反应，在580 nm处（I580 nm）的共振散射强度大大增强。增强强度ΔIRS与IgG浓度在1.3 ~ 1.5×103 ng·mL−1范围内成正比，检测限为0.78 ng·mL−1。该方法灵敏度高，选择性好，可用于人血清中IgG的定量测定，结果满意。
A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti-human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold-labeled goat anti-human IgG and IgG took place, and the resonance scattering intensity at 580 nm (I580 nm) was enhanced greatly. The enhanced intensity ΔIRS is proportional to the IgG concentration from 1.3 to 1.5×103 ng·mL−1, with a detection limit of 0.78 ng·mL−1. This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results.