Quantitative Regional and Ultrastructural Localization of the Cav2.3 Subunit of R-type Calcium Channel in Mouse Brain
Quantitative Regional and Ultrastructural Localization of the Cav2.3 Subunit of R-type Calcium Channel in Mouse Brain
复制标题
DOI：
10.1523/jneurosci.1142-12.2012
复制
发表时间：
2012-09-26
影响因子：
5.3
通讯作者：
Fukazawa, Yugo
中科院分区：
文献类型：
--
作者：
Parajuli, Laxmi Kumar;Nakajima, Chikako;Fukazawa, Yugo
R型钙通道（RTCCs）在突触可塑性中的作用是众所周知的，但对它们在不同神经元隔室中的亚细胞分布知之甚少。使用亚型特异性抗体，我们的特点是区域和亚细胞定位的Ca（V）2.3在小鼠和大鼠在光镜和电镜水平。Ca（v）2.3免疫金颗粒被发现主要是在脚间核突触前，但在其他脑区突触后。海马CA1区电子显微镜图像的连续切片分析显示，与锥体细胞胞体相比，树突状干质膜中的免疫金颗粒密度更高。然而，标记密度之间没有显着差异的顶端，斜，或基底树突。在树突棘的质膜上也观察到免疫金颗粒，包括突触和突触外位点。单个脊柱头包含
R-type calcium channels (RTCCs) are well known for their role in synaptic plasticity, but little is known about their subcellular distribution across various neuronal compartments. Using subtype-specific antibodies, we characterized the regional and subcellular localization of Ca(v)2.3 in mice and rats at both light and electron microscopic levels. Ca(v)2.3 immunogold particles were found to be predominantly presynaptic in the interpeduncular nucleus, but postsynaptic in other brain regions. Serial section analysis of electron microscopic images from the hippocampal CA1 revealed a higher density of immunogold particles in the dendritic shaft plasma membrane compared with the pyramidal cell somata. However, the labeling densities were not significantly different among the apical, oblique, or basal dendrites. Immunogold particles were also observed over the plasma membrane of dendritic spines, including both synaptic and extra-synaptic sites. Individual spine heads contained