Targeting of anti-microbial proteins to the hyphal surface amplifies protection of crop plants against Phytophthora pathogens
Targeting of anti-microbial proteins to the hyphal surface amplifies protection of crop plants against Phytophthora pathogens
复制标题
将抗微生物蛋白靶向菌丝表面可增强作物植物免受疫霉病原体的保护
DOI：
10.1016/j.molp.2021.05.007
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发表时间：
2021
期刊：
影响因子：
27.5
通讯作者：
Dou Daolong
中科院分区：
文献类型：
--
作者：
Zhou Yang;Yang Kun;Yan Qiang;Wang Xiaodan;Cheng Ming;Si Jierui;Xue Xue;Shen Danyu;Jing Maofeng;Tyler Brett M.;Dou Daolong
植物病原体是对世界上商业上重要的农业作物（包括马铃薯和大豆）的持续威胁。目前旨在减少作物损失的战略主要依赖于抗病育种和化学农药，而病原体的快速适应性进化往往可以克服这一点。具有内在抗病性的转基因作物提供了一种有希望的替代品，并将继续得到开发。在这里，我们探索了Phytophthora衍生的PI 3 P（磷脂酰肌醇3-磷酸）作为一种新的控制目标，使用蛋白质结合这种脂质直接分泌的抗微生物肽和蛋白（AMP）的Phytophthora病原体的表面。在转基因烟草、大豆和马铃薯中，通过表达与PI 3 P特异性结合结构域（FYVE）融合的两种AMP（GaFP 1或GaFP 3），显著增强了对不同病原菌分离物的抗性。以大豆病原菌P. sojaea为例，我们证明了FYVE结构域可以在多个独立的试验中增强GAFPs的活性，包括体外、体内和植物中进行的试验。突变分析。大豆中PI 3 K1和PI 3 K2基因的表达证实了GAFPs活性的增强与病原菌中PI 3 P水平相关。总的来说，我们的研究提供了一种新的策略，不仅可以用来赋予许多植物对植物病原体的抗性，而且还可能赋予许多其他种类的具有独特靶标的植物病原体的抗性。
Phytophthorapathogens are a persistent threat to the world's commercially important agricultural crops, including potato and soybean. Current strategies aim at reducing crop losses rely mostly on disease-resistance breeding and chemical pesticides, which can be frequently overcome by the rapid adaptive evolution of pathogens. Transgenic crops with intrinsic disease resistance offer a promising alternative and continue to be developed. Here, we exploredPhytophthora-derived PI3P (phosphatidylinositol 3-phosphate) as a novel control target, using proteins that bind this lipid to direct secreted anti-microbial peptides and proteins (AMPs) to the surface ofPhytophthorapathogens. In transgenicNicotiana benthamiana, soybean, and potato plants, significantly enhanced resistance to different pathogen isolates was achieved by expression of two AMPs (GAFP1 or GAFP3 from the Chinese medicinal herbGastrodia elata) fused with a PI3P-specific binding domain (FYVE). Using the soybean pathogenP.sojaeas an example, we demonstrated that the FYVE domain could boost the activities of GAFPs in multiple independent assays, including those performedin vitro,in vivo, andin planta. Mutational analysis ofP. sojae PI3K1andPI3K2genes of this pathogen confirmed that the enhanced activities of the targeted GAFPs were correlated with PI3P levels in the pathogen. Collectively, our study provides a new strategy that could be used to confer resistance not only toPhytophthorapathogens in many plants but also potentially to many other kinds of plant pathogens with unique targets.