Biological, biochemical and molecular features of Trypanosoma cruzi strains isolated from patients infected through oral transmission during a 2005 outbreak in the state of Santa Catarina, Brazil: its correspondence with the new T. cruzi Taxonomy Consensus (2009)
Biological, biochemical and molecular features of Trypanosoma cruzi strains isolated from patients infected through oral transmission during a 2005 outbreak in the state of Santa Catarina, Brazil: its correspondence with the new T. cruzi Taxonomy Consensus (2009)
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DOI：
10.1590/s0074-02762011000800009
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发表时间：
2011-12-01
期刊：
影响因子：
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通讯作者：
Macedo, Andréa Mara
中科院分区：
文献类型：
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作者：
Andrade, Sonia Gumes;Campos, Rozália Figueira;Macedo, Andréa Mara
我们检查了从巴西圣卡塔里纳（2005年）通过口服传播获得的急性恰加斯病患者中分离的克氏锥虫菌株，以及从有袋动物（Didelphis aurita）和载体（Triatoma tibiamaculata）中获得的两种分离株。这些菌株通过其生物学行为和同工酶谱进行表征，并根据基于离散分型单位的新分类共识（2009）进行基因分型，即T. cruzi基因型I-VI。所有菌株均表现出生物群落II型的生物学行为。在6个分离株，晚高峰的寄生虫血症，超过第20天，提出了双重感染的生物群II + III。同工酶显示Z2或Z1和Z2混合型。使用三种多态性基因（细胞色素氧化酶II、剪接前导基因间区和24 S α rRNA）和kDNA微环的限制性片段长度多态性进行基因分型。基于这些标记，除了四个分离物之外，所有分离物都被表征为T。cruzi II基因型。分离到4个混合种群：SC 90、SC 93和SC 97（T.克鲁济岛cruzi II）和SC 95（T.克鲁济岛cruzi VI）。通过不同的方法获得的结果的比较是必不可少的正确识别的混合人口和主要的血统参与表明，表征通过不同的方法可以提供新的见解寄生虫行为的表型和基因型方面之间的关系。
We examined strains of Trypanosoma cruzi isolated from patients with acute Chagas disease that had been acquired by oral transmission in the state of Santa Catarina, Brazil (2005) and two isolates that had been obtained from a marsupial (Didelphis aurita) and a vector (Triatoma tibiamaculata). These strains were characterised through their biological behaviour and isoenzymic profiles and genotyped according to the new Taxonomy Consensus (2009) based on the discrete typing unities, that is, T. cruzi genotypes I-VI. All strains exhibited the biological behaviour of biodeme type II. In six isolates, late peaks of parasitaemia, beyond the 20th day, suggested a double infection with biodemes II + III. Isoenzymes revealed Z2 or mixed Z1 and Z2 profiles. Genotyping was performed using three polymorphic genes (cytochrome oxidase II, spliced leader intergenic region and 24S alpha rRNA) and the restriction fragment length polymorphism of the kDNA minicircles. Based on these markers, all but four isolates were characterised as T. cruzi II genotypes. Four mixed populations were identified: SC90, SC93 and SC97 (T. cruzi I + T. cruzi II) and SC95 (T. cruzi I + T. cruzi VI). Comparison of the results obtained by different methods was essential for the correct identification of the mixed populations and major lineages involved indicating that characterisation by different methods can provide new insights into the relationship between phenotypic and genotypic aspects of parasite behaviour.