Nanostructured biocomposite substrates by electrospinning and electrospraying for the mineralization of osteoblasts
Nanostructured biocomposite substrates by electrospinning and electrospraying for the mineralization of osteoblasts
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DOI：
10.1016/j.biomaterials.2008.12.079
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发表时间：
2009-04-01
期刊：
影响因子：
14
通讯作者：
Ramakrishna, S.
中科院分区：
文献类型：
--
作者：
Gupta, Deepika;Venugopal, J.;Ramakrishna, S.
纳米技术使纳米结构材料的工程能够满足当前骨替代疗法的挑战。采用静电纺丝和静电喷涂相结合的方法制备了聚乳酸-共聚聚己内酯、明胶和羟基磷灰石（HA）的生物复合纳米纤维支架，为成骨细胞的生长和矿化创造了良好的亲骨环境。在静电纺纳米纤维上电喷涂透明质酸纳米粒子有助于获得理想的细胞附着和增殖的粗糙表面形态，并且比透明质酸混纺纳米纤维具有更好的机械性能。纳米纤维支架的孔径较大，孔隙率可达90%，纤维直径在200 ~ 700 nm之间。利用红外光谱（FTIR）和x射线衍射（XRD）分析表征了纳米纤维支架的官能团和化学结构。通过将人胎儿成骨细胞（hFOB）培养在HA混合和喷涂的PLACL/Gel支架上，并评估其生长、增殖、矿化和酶活性，进行细胞-支架相互作用的研究。MTS、ALP、SEM和ARS研究结果证实，透明质酸喷涂生物复合材料支架不仅具有更好的细胞增殖能力，而且矿化和碱性磷酸酶活性（ALP）也得到了增强，证明电喷涂联合静电纺丝制备的生物复合材料纳米纤维支架更优越、更适合骨组织再生。(C) 2008 Elsevier Ltd版权所有。
Nanotechnology has enabled the engineering of nanostructured materials to meet current challenges in bone replacement therapies. Biocomposite nanofibrous scaffolds of poly(L-lactic acid)-co-poly(epsilon-caprolactone), gelatin and hydroxyapatite (HA) were fabricated by combining the electrospinning and electrospraying techniques in order to create a better osteophilic environment for the growth and mineralization of osteoblasts. Electrospraying of HA nanoparticles on electrospun nanofibers helped to attain rough surface morphology ideal for cell attachment and proliferation and also achieve improved mechanical properties than HA blended nanofibers. Nanofibrous scaffolds showed high pore size and porosity up to 90% with fiber diameter in the range of 200-700 nm. Nanofibrous scaffolds were characterized for their functional groups and chemical structure by FTIR and XRD analysis. Studies on cell-scaffold interaction were carried out by culturing human fetal osteoblast cells (hFOB) on both HA blended and sprayed PLACL/Gel scaffolds and assessing their growth, proliferation, mineralization and enzyme activity. The results of MTS, ALP, SEM and ARS studies confirmed, not only did HA sprayed biocomposite scaffolds showed better cell proliferation but also enhanced mineralization and alkaline phosphatase activity (ALP) proving that electrospraying in combination with electrospinning produced Superior and more suitable biocomposite nanofibrous scaffolds for bone tissue regeneration. (C) 2008 Elsevier Ltd. All rights reserved.