Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds
Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds
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DOI：
10.1038/nbt1418
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发表时间：
2008-07-01
影响因子：
46.9
通讯作者：
Melton, Douglas A.
中科院分区：
文献类型：
--
作者：
Huangfu, Danwei;Maehr, Rene;Melton, Douglas A.
Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase ( HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.
