Evaluation of sickle hemoglobin and desickling agents by falling ball viscometry.
Evaluation of sickle hemoglobin and desickling agents by falling ball viscometry.
复制标题
通过落球粘度测定法评价镰状血红蛋白和除镰剂。
DOI：
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发表时间：
1972
期刊：
影响因子：
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通讯作者：
E. Beutler
中科院分区：
文献类型：
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作者：
N. Paniker;I. Ben;E. Beutler
摘要：落球式粘度计已被用于测量不同程度脱氧情况下镰状血红蛋白溶液粘度的增加。发现此类溶液的粘度随储存而迅速降低。这种变化并非由于高铁血红蛋白的形成或可透析或热稳定辅因子的丢失。2,3 - 二磷酸甘油酸、肌醇六磷酸和磷酸吡哆醛使血红蛋白溶液的粘度增加。高浓度尿素以及较低浓度的氰酸盐和氨基甲酰磷酸使其粘度降低。用落球式粘度计进行的测量可能是评估潜在抗镰变药物的一种有用技术。
Abstract A falling ball viscometer has been used to measure the increased viscosity of sickle hemoglobin solutions at varying degrees of deoxygenation. The viscosity of such solutions was found to decrease rapidly with storage. This change was hot due to methemoglobin formation or loss of a dialyzable or heat-stable cofactor. Viscosity of hemoglobin solutions was increased by 2,3-diphosphoglycerate, in inositolhexaphosphate, and pyridoxal phosphate. It was decreased by high concentrations of urea and lower concentrations of cyanate and carbamyl phosphate. Measurements carried out with a falling ball viscometer may be a useful technique for the evaluation of potential desickling agents.