A speculated ribozyme site in the herpes simplex virus type 1 latency-associated transcript gene is not essential for a wild-type reactivation phenotype.
A speculated ribozyme site in the herpes simplex virus type 1 latency-associated transcript gene is not essential for a wild-type reactivation phenotype.
复制标题
1 型单纯疱疹病毒潜伏相关转录基因中推测的核酶位点对于野生型再激活表型并不是必需的。
DOI：
10.1080/13550280802275912
复制
发表时间：
2008
影响因子：
3.2
通讯作者：
Wechsler,StevenL
中科院分区：
文献类型：
--
作者：
Carpenter,Dale;Singh,Sukhpreet;Osorio,Nelson;Hsiang,Chinhui;Jiang,Xianzhi;Jin,Ling;Jones,Clinton;Wechsler,StevenL
在单纯疱疹病毒1型（HSV-1）在感觉神经元中潜伏期间，LAT（潜伏相关转录本）是唯一大量表达的病毒基因。LAT在HSV-1潜伏-再激活周期中起重要作用，因为LAT缺失突变体具有显著降低的再激活表型。仅基于序列分析，推测LAT编码一种核酶，该核酶在LAT如何增强病毒的再活化表型中起重要作用。由于LAT核酶活性从未被报道，我们决定测试匡威的假设，即，该地区的LAT不编码的核酶功能重要的LAT的能力，以提高再活化表型。我们构建了一个病毒突变体（LAT-Rz），其中推测的核酶共有序列被改变，使没有核酶被编码。我们在这里报告，LAT-RZ在小鼠中有野生型再激活表型，证实了推测的LAT核酶不是刺激小鼠潜伏期再激活周期的主导因素的假设。
During herpes simplex virus-1 (HSV-1) latency in sensory neurons, LAT (latency-associated transcript) is the only abundantly expressed viral gene. LAT plays an important role in the HSV-1 latency-reactivation cycle, because LAT deletion mutants have a significantly decreased reactivation phenotype. Based solely on sequence analysis, it was speculated that LAT encodes a ribozyme that plays an important role in how LAT enhances the virus’ reactivation phenotype. Because LAT ribozyme activity has never been reported, we decided to test the converse hypothesis, namely, that this region of LAT does not encode a ribozyme function important for LAT's ability to enhance the reactivation phenotype. We constructed a viral mutant (LAT-Rz) in which the speculated ribozyme consensus sequence was altered such that no ribozyme was encoded. We report here that LAT-Rz had a wild-type reactivation phenotype in mice, confirming the hypothesis that the speculated LAT ribozyme is not a dominant factor in stimulating the latency-reactivation cycle in mice.