Exercise enhances axonal growth and functional recovery in the regenerating spinal cord
Exercise enhances axonal growth and functional recovery in the regenerating spinal cord
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DOI：
10.1016/j.neuroscience.2006.03.044
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Roberts, B. L.
中科院分区：
文献类型：
--
作者：
Doyle, L. M. F.;Roberts, B. L.
我们研究了增强运动活动是否可以加速轴突生长，这是安圭拉鳗鱼脊柱完全横断后功能显着恢复的基础。脊椎横断面较低的鳗鱼（约为体长的 60%）被饲养在暂养池中，它们在那里不活动，或者以大约一个体长/秒的速度逆着水流不断游动。通过测量不同游泳速度下的尾巴拍打频率来定期评估它们的运动。轴突生长是通过用 1,1'-双十三烷基-3,3,3',3'-四甲基吲哚羰花青高氯酸盐顺行标记来确定的，死后插入脊髓，正好位于火车剖面的嘴部。手术后 20 天，在运动的鱼中，距离横切面尾部超过 2 mm 的标记生长锥显着增多（74.6 +/- 2.3%；cf. 34.5 +/- 1.1%）。 40 天时仍然观察到这种差异（57.9 +/- 0.6% cf. 42.1 +/- 2% > 2 mm），但再生轴突在 120 天时具有相似的最大长度（9.8 +/- 0.3 cf. 7.7 +/- 2.8 mm）。手术后，每条鳗鱼在任何给定的游泳速度下更快地波动其整个身体，从而改变了尾部跳动频率和前进速度之间的线性关系横断前建立。在不活动的动物中，术后前 8 天斜率增加高达 112.5 +/- 27.4%，而在此期间，在运动的鱼中观察到较小的上升 (45.6 +/- 0.5%)。此后，两组动物的斜率逐渐下降到手术前的水平，但运动的鳗鱼在 20 +/- 4 天内恢复，而不活动的鱼则需要 40 +/- 5 天。假手术鱼的运动性能未受到10天连续运动的影响，并且与横断前的幼稚鳗鱼的运动性能相似。这些数据表明，运动活性的提高增强了轴突生长并加速了运动功能的恢复。 (c) 2006 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
We investigated whether enhancing locomotory activity could accelerate the axonal growth underlying the significant recovery of function after a complete spinal transection in the eel, Anguilla. Eels with low spinal transections (at about 60% body length) were kept in holding tanks, where they were inactive, or made to swim continually against a water current at about one body length/s. Their locomotion was periodically assessed by measuring tail beat frequencies at different swimming speeds. Axonal growth was determined from anterograde labeling with 1,1'-diotadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate, inserted postmortem into the spinal cord, just rostra[ to the trainsection.Twenty days after surgery, there were significantly more labeled growth cones more than 2 mm caudal from the transection in the exercised fish (74.6 +/- 2.3%; cf. 34.5 +/- 1.1%). This difference was still observed at 40 days (57.9 +/- 0.6% cf. 42.1 +/- 2% > 2 mm), but the regenerated axons were of similar maximum lengths by 120 days (9.8 +/- 0.3 cf. 7.7 +/- 2.8 mm).After surgery, each eel undulated its whole body faster at any given swimming speed, thus changing the linear relationship between tail beat frequency and forward speed established before transection. The slope increased by up to 112.5 +/- 27.4% over the first 8 days post-surgery in inactive animals, while a smaller rise (45.6 +/- 0.5%) was observed in exercised fish during this period. Thereafter, the slope progressively declined to pre-surgery levels in both groups of animals, but the recovery occurred within 20 +/- 4 days in exercised eels, as opposed to 40 +/- 5 days in inactive fish. The locomotory performance of sham-operated fish was unaffected by 10 days of continual locomotion and remained similar to that of naive eels, pre-transection.These data show that elevated locomotory activity enhances axonal growth and accelerates recovery of locomotory function. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.