RNA interference (RNAi) induction with various types of synthetic oligonucleotide duplexes in cultured human cells
RNA interference (RNAi) induction with various types of synthetic oligonucleotide duplexes in cultured human cells
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DOI：
10.1016/s0014-5793(02)02860-0
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发表时间：
2002-06-19
期刊：
影响因子：
3.5
通讯作者：
Hohjoh, H
中科院分区：
文献类型：
--
作者：
Hohjoh, H
研究了不同类型的合成的Photinus荧光素酶基因寡核苷酸双链对转染人细胞的序列特异性RNA干扰（RNAi）活性的诱导作用。结果表明，与具有脱氧核糖核苷酸3′悬垂的RNA相比，具有核糖核苷酸3′悬垂的RNA双链能诱导更有效的RNAi活性，并且义链DNA/反义链RNA杂交体能诱导中等程度的RNAi活性。这些结果表明，在人类RNAi和无脊椎动物RNAi之间，寡核苷酸双链作为RNAi介质的潜力存在差异，即短干扰rna (sirna)，数据进一步表明，不同的sirna诱导不同水平的RNAi。(C) 2002年由Elsevier Science B.V.代表欧洲生化学会联合会出版。
Various types of synthetic oligonucleotide duplexes against the Photinus luciferase gene were tested on their induction of the sequence-specific RNA interference (RNAi) activity in transfected human cells. Results indicate that RNA duplexes with ribonucleotide 3' overhangs rather than those with deoxyribonucleotide 3' overhangs induce more efficient RNAi activity, and that sense-stranded DNA/antisense-stranded RNA hybrids induce a moderate RNAi activity. These results suggest that there is a difference in the potential of oligonucleotide duplexes to be RNAi mediators, i.e. short interfering RNAs (siRNAs), between human RNAi and invertebrate RNAL The data further show that different siRNAs induce different levels of RNAi. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.